Inflammatory bone loss in experimental periodontitis induced by Aggregatibacter actinomycetemcomitans in interleukin-1 receptor antagonist knockout mice

The interleukin-1 receptor antagonist (IL-1Ra) binds to IL-1 receptors and inhibits IL-1 activity. However, it is not clear whether IL-1Ra plays a protective role in periodontal disease. This study was undertaken to compare experimental periodontitis induced by Aggregatibacter actinomycetemcomitans...

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Veröffentlicht in:	Infection and immunity 2014-05, Vol.82 (5), p.1904-1913
Hauptverfasser:	Izawa, A, Ishihara, Y, Mizutani, H, Kobayashi, S, Goto, H, Okabe, E, Takeda, H, Ozawa, Y, Kamiya, Y, Sugita, Y, Kubo, K, Kamei, H, Kikuchi, T, Mitani, A, Hayashi, J, Nishihara, T, Maeda, H, Noguchi, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Aggregatibacter actinomycetemcomitans - physiology Animals Bacterial Infections Bone Diseases, Metabolic - etiology Bone Resorption - etiology Gene Expression Regulation Inflammation - etiology Interleukin 1 Receptor Antagonist Protein - genetics Interleukin 1 Receptor Antagonist Protein - metabolism Macrophage Colony-Stimulating Factor - genetics Macrophage Colony-Stimulating Factor - metabolism Mice Mice, Knockout Osteoprotegerin - genetics Osteoprotegerin - metabolism Pasteurellaceae Infections - complications Pasteurellaceae Infections - microbiology Periodontitis - complications Periodontitis - microbiology RANK Ligand - genetics RANK Ligand - metabolism
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Zusammenfassung:	The interleukin-1 receptor antagonist (IL-1Ra) binds to IL-1 receptors and inhibits IL-1 activity. However, it is not clear whether IL-1Ra plays a protective role in periodontal disease. This study was undertaken to compare experimental periodontitis induced by Aggregatibacter actinomycetemcomitans in IL-1Ra knockout (KO) mice and wild-type (WT) mice. Computed tomography (CT) analysis and hematoxylin-and-eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining were performed. In addition, osteoblasts were isolated; the mRNA expression of relevant genes was assessed by real-time quantitative PCR (qPCR); and calcification was detected by Alizarin Red staining. Infected IL-1Ra KO mice exhibited elevated (P,
ISSN:	0019-9567 1098-5522
DOI:	10.1128/iai.01618-13