A gene expression study of normal and damaged cartilage in anteromedial gonarthrosis, a phenotype of osteoarthritis

Summary Objective To identify osteoarthritis (OA) relevant genes and pathways in damaged and undamaged cartilage isolated from the knees of patients with anteromedial gonarthrosis (AMG) – a specific form of knee OA. Design Cartilage was obtained from nine patients undergoing unicompartmental knee re...

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Veröffentlicht in:	Osteoarthritis and cartilage 2014-02, Vol.22 (2), p.334-343
Hauptverfasser:	Snelling, S, Rout, R, Davidson, R, Clark, I, Carr, A, Hulley, P.A, Price, A.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Arthroplasty, Replacement, Knee Cartilage Cartilage, Articular - metabolism Disease Progression Female Gene expression Gene Expression Profiling - methods Gene Expression Regulation - physiology Genetic Predisposition to Disease Humans Knee Male Microarray Middle Aged Oligonucleotide Array Sequence Analysis - methods Osteoarthritis, Knee - genetics Osteoarthritis, Knee - metabolism Osteoarthritis, Knee - surgery Phenotype Real-Time Polymerase Chain Reaction - methods Rheumatology Signalling Transcriptome - physiology
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Zusammenfassung:	Summary Objective To identify osteoarthritis (OA) relevant genes and pathways in damaged and undamaged cartilage isolated from the knees of patients with anteromedial gonarthrosis (AMG) – a specific form of knee OA. Design Cartilage was obtained from nine patients undergoing unicompartmental knee replacement (UKR) for AMG. AMG provides a spatial representation of OA progression; showing a reproducible and histologically validated pattern of cartilage destruction such that damaged and undamaged cartilage from within the same knee can be consistently isolated and examined. Gene expression was analysed by microarray and validated using real-time PCR. Results Damaged and undamaged cartilage showed distinct gene expression profiles. 754 genes showed significant up- or down-regulation (non-False discovery rate (FDR) P
ISSN:	1063-4584 1522-9653
DOI:	10.1016/j.joca.2013.12.009