cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma

cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma

cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat--mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro I...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1985-04, Vol.82 (8), p.2460-2464
Hauptverfasser: Martens, C.L, Huff, T.F, Jardieu, P, Trounstine, M.L, Coffman, R.L, Ishizaka, K, Moore, K.W
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
Biotechnology
cell biology
Cell lines
cloning
Cloning, Molecular
Complementary DNA
DNA
DNA - genetics
Fundamental and applied biological sciences. Psychology
genetic code
Genetic engineering
Genetic technics
Glycoproteins - genetics
hybridomas
Hybridomas - immunology
immunoglobulin genes
Lymphokines - genetics
Messenger RNA
Methods. Procedures. Technologies
Mice
Molecular cloning
Oocytes
Plasmids
Prostatic Secretory Proteins
Rats
RNA
rodents
T lymphocytes
T-Lymphocytes - immunology
Yeasts
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Beschreibung
Zusammenfassung:cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat--mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of ≈ 60 and ≈ 11 kDa. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. The results suggest that IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and that the 11-kDa IgE-BF is derived from a 60-kDa precursor.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.8.2460