A luminescent oxygen channeling biosensor that measures small GTPase activation
We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2011-07, Vol.47 (26), p.7521-7523 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Niebel, Björn Weiche, Benjamin Mueller, Alan L Li, Dean Y Karnowski, Nora Famulok, Michael |
| description | We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts. |
| doi_str_mv | 10.1039/c1cc11944c |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2011-07, Vol.47 (26), p.7521-7523 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3961041 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Activated Activation Activation analysis Animals Biosensing Techniques - methods Channeling Enzyme Activation Guidelines Luminescent Measurements Mice Monomeric GTP-Binding Proteins - metabolism Multiplexing NIH 3T3 Cells Oxygen - metabolism Screening Sensors Time Factors |
| title | A luminescent oxygen channeling biosensor that measures small GTPase activation |
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