A luminescent oxygen channeling biosensor that measures small GTPase activation

We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-07, Vol.47 (26), p.7521-7523
Hauptverfasser:	Niebel, Björn, Weiche, Benjamin, Mueller, Alan L, Li, Dean Y, Karnowski, Nora, Famulok, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Activated Activation Activation analysis Animals Biosensing Techniques - methods Channeling Enzyme Activation Guidelines Luminescent Measurements Mice Monomeric GTP-Binding Proteins - metabolism Multiplexing NIH 3T3 Cells Oxygen - metabolism Screening Sensors Time Factors
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Beschreibung
Zusammenfassung:	We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c1cc11944c