Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Rapid one‐step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point‐of‐care scheme is demonstrated herein to site‐specifically immobilize azido‐thrombomodulin on sterilized commercial...

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Veröffentlicht in:	Advanced healthcare materials 2014-01, Vol.3 (1), p.30-35
Hauptverfasser:	Qu, Zheng, Krishnamurthy, Venkat, Haller, Carolyn A., Dorr, Brent M., Marzec, Ulla M., Hurst, Sawan, Hinds, Monica T., Hanson, Stephen R., Liu, David R., Chaikof, Elliot L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkylamines Amines - chemistry Amines - metabolism Aminoacyltransferases - metabolism Animals antifouling Azides - chemistry Azides - metabolism Bacterial Proteins - metabolism biocompatible materials Biological and medical sciences biomedical applications Biotin - chemistry Biotin - metabolism Blood Platelets - chemistry Blood Platelets - metabolism Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - metabolism Cysteine Endopeptidases - metabolism Derivatives Disease Models, Animal Feasibility Fundamental and applied biological sciences. Psychology Fundamental immunology Grafts Health care Heparin - chemistry Heparin - metabolism Heparin - therapeutic use Immobilization Immobilized Proteins - chemistry Immobilized Proteins - metabolism Medical sciences Papio Polyethylene Glycols - chemistry Polyethylene Glycols - metabolism Polytetrafluoroethylene - chemistry Polytetrafluoroethylene - metabolism Staphylococcus aureus - enzymology Surface chemistry Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments thrombomodulin Thrombomodulin - chemistry Thrombomodulin - metabolism Thrombosis Thrombosis - drug therapy Tissue, organ and graft immunology
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Beschreibung
Zusammenfassung:	Rapid one‐step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point‐of‐care scheme is demonstrated herein to site‐specifically immobilize azido‐thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin‐coated grafts in a primate model of acute graft thrombosis.
ISSN:	2192-2640 2192-2659
DOI:	10.1002/adhm.201300110