Visualizing Genomes with Oligopaint FISH Probes
Oligopaint probes are fluorescently labeled, single‐stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provid...
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| Veröffentlicht in: | Current protocols in molecular biology (Print) 2014-01, Vol.105 (1), p.14.23.1-14.23.20 |
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| container_title | Current protocols in molecular biology (Print) |
| container_volume | 105 |
| creator | Beliveau, Brian J. Apostolopoulos, Nicholas Wu, Chao‐ting |
| description | Oligopaint probes are fluorescently labeled, single‐stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D‐FISH, and sample preparation. Curr. Protoc. Mol. Biol. 105:14.23.1‐14.23.20. © 2014 by John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/0471142727.mb1423s105 |
| format | Article |
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| ispartof | Current protocols in molecular biology (Print), 2014-01, Vol.105 (1), p.14.23.1-14.23.20 |
| issn | 1934-3639 1934-3647 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | complex DNA library DNA, Single-Stranded - genetics FISH Genome - genetics Humans In Situ Hybridization, Fluorescence - methods oligonucleotide Oligonucleotide Probes - genetics Oligonucleotides - genetics Oligopaint |
| title | Visualizing Genomes with Oligopaint FISH Probes |
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