Visualizing Genomes with Oligopaint FISH Probes

Oligopaint probes are fluorescently labeled, single‐stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provid...

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Veröffentlicht in:	Current protocols in molecular biology (Print) 2014-01, Vol.105 (1), p.14.23.1-14.23.20
Hauptverfasser:	Beliveau, Brian J., Apostolopoulos, Nicholas, Wu, Chao‐ting
Format:	Artikel
Sprache:	eng
Schlagworte:	complex DNA library DNA, Single-Stranded - genetics FISH Genome - genetics Humans In Situ Hybridization, Fluorescence - methods oligonucleotide Oligonucleotide Probes - genetics Oligonucleotides - genetics Oligopaint
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Oligopaint probes are fluorescently labeled, single‐stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D‐FISH, and sample preparation. Curr. Protoc. Mol. Biol. 105:14.23.1‐14.23.20. © 2014 by John Wiley & Sons, Inc.
ISSN:	1934-3639 1934-3647
DOI:	10.1002/0471142727.mb1423s105