Synthesis of a photocaged tamoxifen for light-dependent activation of Cre-ER recombinase-driven gene modification

We report the design of a water-soluble, quaternized tamoxifen photoprobe and demonstrate its application in light-controlled induction of green fluorescent protein expression via a Cre-ER recombinase system.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2013-05, Vol.49 (43), p.4971-4973
Hauptverfasser:	Inlay, Matthew A, Choe, Veronica, Bharathi, Sophia, Fernhoff, Nathaniel B, Baker, Jr, James R, Weissman, Irving L, Choi, Seok Ki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Chemical communication Estrogen Antagonists - chemistry Estrogen Antagonists - pharmacology Estrogen Antagonists - radiation effects Green Fluorescent Proteins - genetics Integrases - genetics Mice Nitrobenzenes - chemistry Nitrobenzenes - radiation effects Receptors, Estrogen - genetics Recombination, Genetic Tamoxifen - chemistry Tamoxifen - pharmacology Tamoxifen - radiation effects Ultraviolet Rays
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Beschreibung
Zusammenfassung:	We report the design of a water-soluble, quaternized tamoxifen photoprobe and demonstrate its application in light-controlled induction of green fluorescent protein expression via a Cre-ER recombinase system.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c3cc42179a