Interactive effects of in vitro binge-like alcohol and ATP on umbilical endothelial nitric oxide synthase post-translational modifications and redox modulation

•Chronic in vitro binge alcohol exposure dysregulates the exquisite coordination and regulation of umbilical endothelial vascular adaptations.•Binge-like alcohol has detrimental effects on all four major eNOS multi-site phosphorylation sites.•There is an interactive effect of alcohol and the endogen...

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Veröffentlicht in:	Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2014-01, Vol.43, p.94-101
Hauptverfasser:	Subramanian, Kaviarasan, Naik, Vishal D., Sathishkumar, Kunju, Sawant, Onkar B., Washburn, Shannon E., Wu, Guoyao, Yallampalli, Chandra, Saade, George R., Hankins, Gary D., Ramadoss, Jayanth
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - pharmacology ATP Binge Drinking - metabolism Cells, Cultured Endothelium eNOS Ethanol - pharmacology FASD Glutathione - metabolism Glutathione Disulfide - metabolism Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - metabolism Humans Nitric Oxide Synthase Type III - metabolism Oxidation-Reduction Protein Processing, Post-Translational - drug effects Umbilical Cord - metabolism Vascular
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Zusammenfassung:	•Chronic in vitro binge alcohol exposure dysregulates the exquisite coordination and regulation of umbilical endothelial vascular adaptations.•Binge-like alcohol has detrimental effects on all four major eNOS multi-site phosphorylation sites.•There is an interactive effect of alcohol and the endogenous eNOS activating agonist ATP on eNOS multi-site phosphorylation state.•Alcohol modulates eNOS-related redox switch post-translational modifications.•Major role for the reproductive vascular system in pathogenesis of Fetal Alcohol Spectrum Disorders. Alcohol dysregulates the regulation of reproductive vascular adaptations. We herein investigated chronic in vitro binge-like alcohol effects on umbilical endothelial nitric oxide synthase (eNOS) multi-site phosphorylation and related redox switches under basal (unstimulated) and stimulated (with ATP) states. Alcohol decreased endothelial excitatory Pser1177eNOS (P
ISSN:	0890-6238 1873-1708
DOI:	10.1016/j.reprotox.2013.11.006