Construction and identification of the pshRNA-CACNA1G-SH-SY5Ycells targeted to silence Cav3.1 mRNA expression

T-type calcium channels are a class of low voltage-dependent calcium channels that may be activated following minor depolarizations of the cell membrane. Cav3.1 is the dominant subtype of the T-type calcium channel in SH-SY5Y cells. T-type channels play a key role in the regulation of the intracellu...

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Veröffentlicht in:	Biomedical reports 2013-07, Vol.1 (4), p.669-673
Hauptverfasser:	WEN, XIANJIE, XU, SHIYUAN, LIU, HONGZHEN, LIANG, HUA, YANG, CHENXIANG, WANG, HANBING
Format:	Artikel
Sprache:	eng
Schlagworte:	Anesthetics Annealing Biotechnology Calcium Calcium (intracellular) Calcium channels Calcium channels (N-type) Calcium channels (T-type) Calcium channels (voltage-gated) Cav3.1 Cell culture Channels Construction methods Deoxyribonucleic acid Depolarization Design DNA DNA polymerase Gene expression Genetic engineering Inhibitors Local anesthetics Low voltage Neurotoxicity Plasmids Polymerase chain reaction Protein expression Proteins Reverse transcription reverse transcription-polymerase chain reaction Ribonucleic acid RNA RNA interference RNA polymerase RNA-mediated interference Selectivity Silence T-type calcium channel Transfection Vectors (Biology) western blot analysis
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