Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

Engineered metabolic pathways often suffer from flux imbalances that can overburden the cell and accumulate intermediate metabolites, resulting in reduced product titers. One way to alleviate such imbalances is to adjust the expression levels of the constituent enzymes using a combinatorial expressi...

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Veröffentlicht in:	Nucleic acids research 2013-12, Vol.41 (22), p.10668-10678
Hauptverfasser:	Lee, Michael E, Aswani, Anil, Han, Audrey S, Tomlin, Claire J, Dueber, John E
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosynthetic Pathways - genetics Gene Expression Regulation Gene Library Genotyping Techniques Indoles - metabolism Linear Models Metabolic Engineering - methods Promoter Regions, Genetic Protein Biosynthesis Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Synthetic Biology and Chemistry
Online-Zugang:	Volltext
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