Mammalian Cells with Altered Forms of RNA Polymerase II

Mammalian Cells with Altered Forms of RNA Polymerase II

Mutants of Chinese hamster ovary cells that are resistant to α -amanitin can be isolated. At least some of these mutants contain an altered form of DNA-dependent RNA polymerase II, as indicated by its resistance to α -amanitin. These results indicate that mutation to α -amanitin resistance involves...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1972-11, Vol.69 (11), p.3119-3123
Hauptverfasser: Chan, V. L., Whitmore, G. F., Siminovitch, Louis
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological Sciences: Cell Biology
Cell culture techniques
Cell growth
Cell Line
Cell lines
Cells
CHO cells
Chromatography, DEAE-Cellulose
Cricetinae
DNA
DNA - metabolism
DNA-Directed RNA Polymerases - analysis
DNA-Directed RNA Polymerases - antagonists & inhibitors
DNA-Directed RNA Polymerases - isolation & purification
Drug Resistance
Enzymes
Female
Mycotoxins - pharmacology
Ovaries
Ovary - drug effects
Ovary - enzymology
RNA
Somatic cells
Templates, Genetic
Tritium
Uracil Nucleotides - metabolism
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Zusammenfassung:Mutants of Chinese hamster ovary cells that are resistant to α -amanitin can be isolated. At least some of these mutants contain an altered form of DNA-dependent RNA polymerase II, as indicated by its resistance to α -amanitin. These results indicate that mutation to α -amanitin resistance involves a change of a structural gene.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.11.3119