A cell rolling cytometer reveals the correlation between mesenchymal stem cell dynamic adhesion and differentiation state

A cell rolling cytometer reveals the correlation between mesenchymal stem cell dynamic adhesion and differentiation state

This communication presents quantitative studies of the dynamic adhesion behavior of mesenchymal stem cells (MSCs) enabled by the combination of cell-surface receptor-ligand interactions and three-dimensional hydrodynamic control by microtopography. Quantifying weak adhesion of mesenchymal stem cell...

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Veröffentlicht in:Lab on a chip 2014-01, Vol.14 (1), p.161-166
Hauptverfasser: Choi, Sungyoung, Levy, Oren, Coelho, Mónica B, Cabral, Joaquim M. S, Karp, Jeffrey M, Karnik, Rohit
Format: Artikel
Sprache:eng
Schlagworte:
Adhesion
Adipocytes - cytology
Cell Adhesion
Cell Differentiation
Cells, Cultured
Differentiation
Dynamic tests
Dynamics
Flow Cytometry - instrumentation
Flow Cytometry - methods
Fluid dynamics
Fluid flow
HL-60 Cells
Humans
Ligands
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Osteoblasts - cytology
Phenotype
Receptors, Cell Surface - chemistry
Receptors, Cell Surface - metabolism
Stem cells
Three dimensional
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Beschreibung
Zusammenfassung:This communication presents quantitative studies of the dynamic adhesion behavior of mesenchymal stem cells (MSCs) enabled by the combination of cell-surface receptor-ligand interactions and three-dimensional hydrodynamic control by microtopography. Quantifying weak adhesion of mesenchymal stem cells enables detection of phenotype alterations, potentially enabling quality control for cell-based therapies.
ISSN:1473-0197
1473-0189
DOI:10.1039/c3lc50923k