Slowing DNA Translocation through a Nanopore Using a Functionalized Electrode

Slowing DNA Translocation through a Nanopore Using a Functionalized Electrode

Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoeth...

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Veröffentlicht in:ACS nano 2013-11, Vol.7 (11), p.10319-10326
Hauptverfasser: Krishnakumar, Padmini, Gyarfas, Brett, Song, Weisi, Sen, Suman, Zhang, Peiming, Krstić, Predrag, Lindsay, Stuart
Format: Artikel
Sprache:eng
Schlagworte:
Biological Transport
Coating
Deoxyribonucleic acid
DNA - chemistry
DNA, Single-Stranded - chemistry
Electrodes
Equipment Design
Hydrogen Bonding
Materials Testing
Mathematical analysis
Membranes, Artificial
Microscopy, Atomic Force
Monolayers
Nanopores
Nanostructure
Nanotechnology - methods
Normal Distribution
Palladium
Palladium - chemistry
Porosity
Retarding
Sulfhydryl Compounds - chemistry
Surface Properties
Time Factors
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Zusammenfassung:Nanopores were fabricated with an integrated microscale Pd electrode coated with either a hydrogen-bonding or hydrophobic monolayer. Bare pores, or those coated with octanethiol, translocated single-stranded DNA with times of a few microseconds per base. Pores functionalized with 4(5)-(2-mercaptoethyl)-1H-imidazole-2-carboxamide slowed average translocation times, calculated as the duration of the event divided by the number of bases translocated, to about 100 μs per base at biases in the range of 50 to 80 mV.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn404743f