The Archaeal Exosome: Identification and Quantification of Site-Specific Motions That Correlate with Cap and RNA Binding

Big and unexpectedly flexible: The 173 kDa exosome core is unexpectedly dynamic in solution. The kinetic and thermodynamic properties connected with the identified exchange process were quantified. Both cap protein and RNA substrate binding significantly alter the identified motions, suggesting that...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Angewandte Chemie International Edition 2013-08, Vol.52 (32), p.8312-8316
Hauptverfasser:	Audin, Maxime J. C., Dorn, Georg, Fromm, Simon A., Reiss, Kerstin, Schütz, Stefan, Vorländer, Matthias K., Sprangers, Remco
Format:	Artikel
Sprache:	eng
Schlagworte:	Archaea - genetics Binding Communications Correlation Dynamics Exchange exosome Exosomes - genetics Magnetic Resonance Spectroscopy molecular machines NMR spectroscopy Protein Binding protein dynamics Proteins Ribonucleic acids RNA - metabolism Thermodynamic properties
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Big and unexpectedly flexible: The 173 kDa exosome core is unexpectedly dynamic in solution. The kinetic and thermodynamic properties connected with the identified exchange process were quantified. Both cap protein and RNA substrate binding significantly alter the identified motions, suggesting that these interactions occur through conformational selection.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201302811