Engineering New Protein-Protein Interactions on the β-Propeller Fold by Yeast Cell Surface Display

Reinventing the wheel: The β‐propeller domain folds like a wheel to provide key protein–protein interactions in the cell. Here we used high‐throughput yeast sorting to “invent” β‐propellers of new binding specificities with cellular targets.

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Bibliographische Detailangaben
Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2013-03, Vol.14 (4), p.426-430
Hauptverfasser:	Zhang, Keya, Li, Heng, Bhuripanyo, Karan, Zhao, Bo, Chen, Tiffany F., Zheng, Ning, Yin, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing - chemistry Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism Animals beta-propeller folds Cell Surface Display Techniques - methods Cytoskeletal Proteins - chemistry Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism enzymes genetic engineering Kelch repeats Kelch-Like ECH-Associated Protein 1 Mice Models, Molecular Mutation Protein Folding Protein Interaction Maps Protein Structure, Secondary Protein Structure, Tertiary yeast-cell-surface display Yeasts - genetics Yeasts - metabolism
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Beschreibung
Zusammenfassung:	Reinventing the wheel: The β‐propeller domain folds like a wheel to provide key protein–protein interactions in the cell. Here we used high‐throughput yeast sorting to “invent” β‐propellers of new binding specificities with cellular targets.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.201200718