Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas

CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates...

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Veröffentlicht in:	Genetics (Austin) 2013-11, Vol.195 (3), p.1167-1171
Hauptverfasser:	Chiu, Hui, Schwartz, Hillel T, Antoshechkin, Igor, Sternberg, Paul W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Caenorhabditis elegans Caenorhabditis elegans - genetics CRISPR-Cas Systems Genes Genes, Helminth Genetic Engineering Genome, Helminth Genomes Genomics Humans Mutation Nematoda Nematodes Ribonucleic acid RNA RNA Editing - genetics RNA, Helminth - genetics
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Zusammenfassung:	CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.
ISSN:	1943-2631 0016-6731 1943-2631
DOI:	10.1534/genetics.113.155879