Combinatorial Domain Swaps Provide Insights into the Rules of Fungal Polyketide Synthase Programming and the Rational Synthesis of Non-Native Aromatic Products

Combinatorial Domain Swaps Provide Insights into the Rules of Fungal Polyketide Synthase Programming and the Rational Synthesis of Non-Native Aromatic Products

Playing by the rules: Combinatorial domain swaps among “deconstructed” non‐reducing polyketide synthases (NR‐PKSs) revealed the rules behind product assembly (see scheme). The control exerted by individual catalytic domains was found to be sufficiently great that heterocombinations of domains from d...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2013-02, Vol.52 (6), p.1718-1721
Hauptverfasser: Vagstad, Anna L., Newman, Adam G., Storm, Philip A., Belecki, Katherine, Crawford, Jason M., Townsend, Craig A.
Format: Artikel
Sprache:eng
Schlagworte:
Assembly
Catalysis
Catalysts
Catalytic Domain
Combinatorial analysis
Cyclization
domain swapping
Fungi
Fungi - enzymology
Metabolic Engineering
natural products
pathway engineering
Polyketide Synthases - chemistry
Polyketide Synthases - metabolism
polyketides
Programming
Synthesis
Transaldolase - chemistry
Transaldolase - metabolism
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Zusammenfassung:Playing by the rules: Combinatorial domain swaps among “deconstructed” non‐reducing polyketide synthases (NR‐PKSs) revealed the rules behind product assembly (see scheme). The control exerted by individual catalytic domains was found to be sufficiently great that heterocombinations of domains from different NR‐PKSs synthesized products in a predictable manner.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201208550