Hofmeister Salts Recover a Misfolded Multiprotein Complex for Subsequent Structural Measurements in the Gas Phase

A misfolded protein complex (see picture, red tetramer), which exists both in solution and in the gas phase, can be recovered back to a native‐like structure (blue) by the addition of salts prior to desorption/ionization into the gas phase using nano‐electrospray ionization.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Angewandte Chemie International Edition 2013-08, Vol.52 (32), p.8329-8332
Hauptverfasser:	Han, Linjie, Ruotolo, Brandon T.
Format:	Artikel
Sprache:	eng
Schlagworte:	circular dichroism Desorption differential scanning calorimetry Gas phases Gases - chemistry ion mobility Ionization mass spectrometry Multiprotein Complexes - chemistry Nanostructure Pictures Protein Conformation protein misfolding Proteins Proteins - chemistry Salts - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A misfolded protein complex (see picture, red tetramer), which exists both in solution and in the gas phase, can be recovered back to a native‐like structure (blue) by the addition of salts prior to desorption/ionization into the gas phase using nano‐electrospray ionization.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201301893