Rewiring Translation for Elongation Factor Tu-Dependent Selenocysteine Incorporation

Enjoying UTu in concert: A synthetic tRNA (tRNAUTu) was used as a substrate for three E. coli proteins: seryl‐tRNA synthetase (SerRS) forming Ser‐tRNAUTu, selenocysteine (Sec) synthase (SelA) generating Sec‐tRNAUTu, and EF‐Tu for Sec‐tRNAUTu transport to the ribosome (see scheme). tRNAUTu can be use...

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Veröffentlicht in:	Angewandte Chemie International Edition 2013-01, Vol.52 (5), p.1441-1445
Hauptverfasser:	Aldag, Caroline, Bröcker, Markus J., Hohn, Michael J., Prat, Laure, Hammond, Gifty, Plummer, Abigail, Söll, Dieter
Format:	Artikel
Sprache:	eng
Schlagworte:	Concerts Elongation Formate Dehydrogenases - chemistry Formate Dehydrogenases - genetics Formate Dehydrogenases - metabolism genetic code Glutathione Peroxidase - chemistry Glutathione Peroxidase - genetics Glutathione Peroxidase - metabolism Humans Hydrogenase - chemistry Hydrogenase - genetics Hydrogenase - metabolism Insertion Multienzyme Complexes - chemistry Multienzyme Complexes - genetics Multienzyme Complexes - metabolism orthogonal translation Peptide Elongation Factor Tu - chemistry Peptide Elongation Factor Tu - metabolism Peroxidase Protein Biosynthesis Protein Engineering Proteins Rewiring Ribosomes - metabolism RNA, Transfer - chemistry RNA, Transfer - metabolism selenocysteine Selenocysteine - metabolism synthetic biology Translations Transport
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Beschreibung
Zusammenfassung:	Enjoying UTu in concert: A synthetic tRNA (tRNAUTu) was used as a substrate for three E. coli proteins: seryl‐tRNA synthetase (SerRS) forming Ser‐tRNAUTu, selenocysteine (Sec) synthase (SelA) generating Sec‐tRNAUTu, and EF‐Tu for Sec‐tRNAUTu transport to the ribosome (see scheme). tRNAUTu can be used by the ribosome, thus allowing site‐specific Sec insertion into proteins, including formate dehydrogenase H, selenoglutaredoxin, and glutathione peroxidase.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201207567