Protoribosome by quantum kernel energy method

Experimental evidence suggests the existence of an RNA molecular prebiotic entity, called by us the “protoribosome,” which may have evolved in the RNA world before evolution of the genetic code and proteins. This vestige of the RNA world, which possesses all of the capabilities required for peptide...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2013-09, Vol.110 (37), p.14900-14905
Hauptverfasser: Huang, Lulu, Krupkin, Miri, Bashan, Anat, Yonath, Ada, Massa, Lou
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Sprache:eng
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Zusammenfassung:Experimental evidence suggests the existence of an RNA molecular prebiotic entity, called by us the “protoribosome,” which may have evolved in the RNA world before evolution of the genetic code and proteins. This vestige of the RNA world, which possesses all of the capabilities required for peptide bond formation, seems to be still functioning in the heart of all of the contemporary ribosome. Within the modern ribosome this remnant includes the peptidyl transferase center. Its highly conserved nucleotide sequence is suggestive of its robustness under diverse environmental conditions, and hence on its prebiotic origin. Its twofold pseudosymmetry suggests that this entity could have been a dimer of self-folding RNA units that formed a pocket within which two activated amino acids might be accommodated, similar to the binding mode of modern tRNA molecules that carry amino acids or peptidyl moieties. Using quantum mechanics and crystal coordinates, this work studies the question of whether the putative protoribosome has properties necessary to function as an evolutionary precursor to the modern ribosome. The quantum model used in the calculations is density functional theory–B3LYP/3–21G*, implemented using the kernel energy method to make the computations practical and efficient. It occurs that the necessary conditions that would characterize a practicable protoribosome—namely (i) energetic structural stability and (ii) energetically stable attachment to substrates—are both well satisfied.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1314112110