Decoding in the Absence of a Codon by tmRNA and SmpB in the Ribosome

In bacteria, ribosomes stalled at the end of truncated messages are rescued by transfer-messenger RNA (tmRNA), a bifunctional molecule that acts as both a transfer RNA (tRNA) and a messenger RNA (mRNA), and SmpB, a small protein that works in concert with tmRNA. Here, we present the crystal structur...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2012-03, Vol.335 (6074), p.1366-1369
Hauptverfasser:	Neubauer, Cajetan, Gillet, Reynald, Kelley, Ann C., Ramakrishnan, V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anticodon bacteria Bacterial Proteins - chemistry Bacterial Proteins - metabolism Base Sequence Biochemistry, Molecular Biology Biological and medical sciences Codons Crystal structure Crystalline structure Crystallography, X-Ray Fundamental and applied biological sciences. Psychology Gram-negative bacteria Life Sciences Messenger RNA Models, Molecular Molecular biophysics Molecular chemistry Molecular Sequence Data Nucleic Acid Conformation Nucleotides Open reading frames Peptide Elongation Factor Tu - chemistry Peptide Elongation Factor Tu - metabolism Peptides Protein Biosynthesis Protein Conformation Proteins Ribonucleic acid Ribosome Subunits, Small, Bacterial - chemistry Ribosome Subunits, Small, Bacterial - metabolism Ribosome Subunits, Small, Bacterial - ultrastructure Ribosomes Ribosomes - chemistry Ribosomes - metabolism Ribosomes - ultrastructure RNA RNA, Bacterial - chemistry RNA, Bacterial - metabolism RNA, Messenger - chemistry RNA, Messenger - metabolism RNA, Transfer - chemistry RNA, Transfer - metabolism RNA-Binding Proteins - chemistry RNA-Binding Proteins - metabolism Structure in molecular biology Thermus thermophilus - chemistry Thermus thermophilus - genetics Thermus thermophilus - metabolism Thermus thermophilus - ultrastructure Transfer RNA Translation translation (genetics)
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Zusammenfassung:	In bacteria, ribosomes stalled at the end of truncated messages are rescued by transfer-messenger RNA (tmRNA), a bifunctional molecule that acts as both a transfer RNA (tRNA) and a messenger RNA (mRNA), and SmpB, a small protein that works in concert with tmRNA. Here, we present the crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 angstroms resolution. The structure shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon in the A site of the ribosome and explains why the tmRNA-SmpB system does not interfere with normal translation.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1217039