Artificial repressors for controlling gene expression in bacteria

Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repre...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2013-01, Vol.49 (39), p.4325-4327
Hauptverfasser:	Politz, Mark C, Copeland, Matthew F, Pfleger, Brian F
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Chemical communication DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Lac Repressors - genetics Lac Repressors - metabolism Promoter Regions, Genetic Transcription, Genetic Xanthomonas - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c2cc37107c