Combining genome-wide methods to investigate the genetic complexity of courtship song variation in Drosophila melanogaster
Little is currently known about the genetic complexity of quantitative behavioral variation, the types of genes involved, or their effects on intermediate phenotypes. Here, we conduct a genome-wide association study of Drosophila melanogaster courtship song variation using 168 sequenced inbred lines...
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| Veröffentlicht in: | Molecular biology and evolution 2013-09, Vol.30 (9), p.2113-2120 |
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| creator | Turner, Thomas L Miller, Paige M Cochrane, Veronica A |
| description | Little is currently known about the genetic complexity of quantitative behavioral variation, the types of genes involved, or their effects on intermediate phenotypes. Here, we conduct a genome-wide association study of Drosophila melanogaster courtship song variation using 168 sequenced inbred lines, and fail to find highly significant associations. However, by combining these data with results from a well-powered Evolve and Resequence (E&R) study on the same trait, we provide statistical evidence that some power to associate genotype and phenotype is available. Genes that are significant in both analyses are enriched for expression in the nervous system, and affect neural development and synaptic growth when perturbed. Quantitative complementation at one of these loci, Syntrophin-like 1, supports a hypothesis that variation at this locus affects variation in the inter-pulse interval of courtship song. These results suggest that experimental evolution may provide an approach for genome-scale replication in Drosophila. |
| doi_str_mv | 10.1093/molbev/mst111 |
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Here, we conduct a genome-wide association study of Drosophila melanogaster courtship song variation using 168 sequenced inbred lines, and fail to find highly significant associations. However, by combining these data with results from a well-powered Evolve and Resequence (E&R) study on the same trait, we provide statistical evidence that some power to associate genotype and phenotype is available. Genes that are significant in both analyses are enriched for expression in the nervous system, and affect neural development and synaptic growth when perturbed. Quantitative complementation at one of these loci, Syntrophin-like 1, supports a hypothesis that variation at this locus affects variation in the inter-pulse interval of courtship song. These results suggest that experimental evolution may provide an approach for genome-scale replication in Drosophila.</description><identifier>ISSN: 0737-4038</identifier><identifier>EISSN: 1537-1719</identifier><identifier>DOI: 10.1093/molbev/mst111</identifier><identifier>PMID: 23777628</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Animals ; Calcium-Binding Proteins - genetics ; Cell Adhesion Molecules - genetics ; Courtship ; Discoveries ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophila Proteins - genetics ; Evolution ; Evolution, Molecular ; Female ; Genes ; Genes, Insect ; Genetic Variation ; Genome, Insect ; Genome-Wide Association Study ; Genotype ; Genotype & phenotype ; Insects ; Male ; Membrane Proteins - genetics ; Muscle Proteins - genetics ; Nerve Tissue Proteins - genetics ; Nervous system ; Phenotype ; Quantitative Trait Loci ; Vocalization, Animal - physiology</subject><ispartof>Molecular biology and evolution, 2013-09, Vol.30 (9), p.2113-2120</ispartof><rights>Copyright Oxford Publishing Limited(England) Sep 2013</rights><rights>The Author 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-fa8cc45aeb2fe9760ee76137ad669be4929ea86707f6d33e0b679df598eaf14b3</citedby><cites>FETCH-LOGICAL-c448t-fa8cc45aeb2fe9760ee76137ad669be4929ea86707f6d33e0b679df598eaf14b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748354/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748354/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23777628$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turner, Thomas L</creatorcontrib><creatorcontrib>Miller, Paige M</creatorcontrib><creatorcontrib>Cochrane, Veronica A</creatorcontrib><title>Combining genome-wide methods to investigate the genetic complexity of courtship song variation in Drosophila melanogaster</title><title>Molecular biology and evolution</title><addtitle>Mol Biol Evol</addtitle><description>Little is currently known about the genetic complexity of quantitative behavioral variation, the types of genes involved, or their effects on intermediate phenotypes. Here, we conduct a genome-wide association study of Drosophila melanogaster courtship song variation using 168 sequenced inbred lines, and fail to find highly significant associations. However, by combining these data with results from a well-powered Evolve and Resequence (E&R) study on the same trait, we provide statistical evidence that some power to associate genotype and phenotype is available. Genes that are significant in both analyses are enriched for expression in the nervous system, and affect neural development and synaptic growth when perturbed. Quantitative complementation at one of these loci, Syntrophin-like 1, supports a hypothesis that variation at this locus affects variation in the inter-pulse interval of courtship song. These results suggest that experimental evolution may provide an approach for genome-scale replication in Drosophila.</description><subject>Animals</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Courtship</subject><subject>Discoveries</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins - genetics</subject><subject>Evolution</subject><subject>Evolution, Molecular</subject><subject>Female</subject><subject>Genes</subject><subject>Genes, Insect</subject><subject>Genetic Variation</subject><subject>Genome, Insect</subject><subject>Genome-Wide Association Study</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Insects</subject><subject>Male</subject><subject>Membrane Proteins - genetics</subject><subject>Muscle Proteins - genetics</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nervous system</subject><subject>Phenotype</subject><subject>Quantitative Trait Loci</subject><subject>Vocalization, Animal - physiology</subject><issn>0737-4038</issn><issn>1537-1719</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks9vFCEUx4nR2G316NWQePEyFgaGHxcTs1o1aeKlPRNm5s0MzQDjwG6tf71stjbaU09A-PDJe18eQm8o-UCJZuc-zi3sz33KlNJnaEMbJisqqX6ONkSWPSdMnaDTlG4IoZwL8RKd1ExKKWq1Qb-30bcuuDDiEUL0UN26HrCHPMU-4RyxC3tI2Y02A84THDDIrsNd9MsMv1y-w3Eop92a0-QWnGJx7e3qbHYxlOf48xpTXCY32-KdbYijTRnWV-jFYOcEr-_XM3R98eVq-626_PH1-_bTZdVxrnI1WNV1vLHQ1gNoKQiAFJRJ2wuhW-C61mCVkEQOomcMSCuk7odGK7AD5S07Qx-P3mXXeug7CHm1s1lW5-16Z6J15v-b4CYzxr1hkivW8CJ4fy9Y489dCcN4lzqYSysQd8lQznRd64Y0T0BrKUvJ5GB99wi9KRmGksSBUkJp3YhCVUeqKyGmFYaHuikxhwEwxwEwxwEo_Nt_m32g__44-wNOVrJb</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Turner, Thomas L</creator><creator>Miller, Paige M</creator><creator>Cochrane, Veronica A</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130901</creationdate><title>Combining genome-wide methods to investigate the genetic complexity of courtship song variation in Drosophila melanogaster</title><author>Turner, Thomas L ; 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| subjects | Animals Calcium-Binding Proteins - genetics Cell Adhesion Molecules - genetics Courtship Discoveries Drosophila melanogaster Drosophila melanogaster - genetics Drosophila Proteins - genetics Evolution Evolution, Molecular Female Genes Genes, Insect Genetic Variation Genome, Insect Genome-Wide Association Study Genotype Genotype & phenotype Insects Male Membrane Proteins - genetics Muscle Proteins - genetics Nerve Tissue Proteins - genetics Nervous system Phenotype Quantitative Trait Loci Vocalization, Animal - physiology |
| title | Combining genome-wide methods to investigate the genetic complexity of courtship song variation in Drosophila melanogaster |
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