Probing RNA Structure with Chemical Reagents and Enzymes

This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes,...

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Veröffentlicht in:	Current Protocols in Nucleic Acid Chemistry 2001-05, Vol.Chapter 6 (1), p.6.1.1-6.1.21
Hauptverfasser:	Ziehler, William A., Engelke, David R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehydes - chemistry Bacteriophage T4 - enzymology Base Pairing Biochemistry - methods Butanones CME-Carbodiimide - analogs & derivatives CME-Carbodiimide - chemistry Diethyl Pyrocarbonate - chemistry Electrophoresis Endonucleases - metabolism Ethylnitrosourea - chemistry Indicators and Reagents Nucleic Acid Conformation - drug effects Phosphorus Radioisotopes Polynucleotide 5'-Hydroxyl-Kinase - metabolism Reference Standards RNA - chemistry RNA - metabolism Sulfuric Acid Esters - chemistry
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Beschreibung
Zusammenfassung:	This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.
ISSN:	1934-9270 1934-9289
DOI:	10.1002/0471142700.nc0601s00