Antioxidant and anti-dermatophytic properties leaf and stem bark of Xylosma longifolium clos

The present study was carried out to assess the phytochemical and anti-dermatophytic effect of the leaf and bark extracts of Xylosma longifolium Clos. The leaf and stem bark are used by the indigenous people of Manipur, India for treatment of skin diseases. The leaves and stem barks of Xylosma longifolium were extracted using petroleum ether, chloroform and methanol respectively. The different extracts of each plant parts were tested for antioxidant activity using DPPH assay. The phenolic content was assayed using Folin-Ciocalteu colorimetric method. Each extracts was further analysed by RP-HPLC to quantify some individual flavonoid components. The anti-dermatophytic activity was evaluated both by agar diffusion method and micro wells dilution method against the Microsporum boullardii MTCC 6059, M. canis (MTCC 2820 and MTCC 32700), M. gypseum MTCC 2819, Trichophyton ajelloi MTCC 4878, T. rubrum (MTCC 296 and MTCC 3272). The free radical scavenging activity values were ranged from 0.7 to 1.41 mg/ml and 0.6 to 1.23 mg/ml, respectively for leaf and stem bark extracts. The amount of total phenolic contents of the extracts occurred in both leaf and bark in the range of 12 to 56.6 mg GAE/100 g and 16 to 58 mg GAE/100 g respectively. RP-HPLC analysis for flavonoids revealed the presence of two major flavonoid compounds, rutin and catechin. Kaempferol was in trace or absent. Methanol leaf extract showed significant low inhibitory effect against tested fungus Trichophyton ajelloi MTCC 4878 (0.140625 mg/ml) as the most sensitive. These finding suggest that the methanol leaf extract tested contain compounds with antimicrobial properties. The results of our study may partially justify the folkloric uses on the plant studied and further provide an evidence that the leaf extract of Xylosma longifolium might be indeed a potential sources of antimicrobial agents.