PI(4,5)P2 DEPENDENT AND Ca2+-REGULATED ER-PLASMA MEMBRANE INTERACTIONS MEDIATED BY THE EXTENDED-SYNAPTOTAGMINS
Most available information on ER-plasma membrane (PM) contacts in cells of higher eukaryotes concerns proteins implicated in the regulation of Ca 2+ entry. However, growing evidence suggests that such contacts play more general roles in cell physiology, pointing to the existence of additionally ubiq...
Gespeichert in:
| Veröffentlicht in: | Cell 2013-06, Vol.153 (7), p.1494-1509 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1509 |
|---|---|
| container_issue | 7 |
| container_start_page | 1494 |
| container_title | Cell |
| container_volume | 153 |
| creator | Giordano, Francesca Saheki, Yasunori Idevall-Hagren, Olof Colombo, Sara Pirruccello, Michelle Milosevic, Ira Gracheva, Elena O. Bagriantsev, Sviatoslav N. Borgese, Nica Camilli, Pietro De |
| description | Most available information on ER-plasma membrane (PM) contacts in cells of higher eukaryotes concerns proteins implicated in the regulation of Ca
2+
entry. However, growing evidence suggests that such contacts play more general roles in cell physiology, pointing to the existence of additionally ubiquitously expressed ER-PM tethers. Here we show that the three Extended-Synaptotagmins (E-Syts) are ER proteins that participate in such tethering function via C2 domain-dependent interactions with the PM that require PI(4,5)P
2
in the case of E-Syt2 and E-Syt3 and also elevation of cytosolic Ca
2+
in the case of E-Syt1. As they form heteromeric complexes, the E-Syts confer cytosolic Ca
2+
regulation to ER-PM contact formation. E-Syts-dependent contacts, however, are not required for store-operated Ca
2+
entry. Thus, the ER-PM tethering function of the E-Syts (tricalbins in yeast), mediate the formation of ER-PM contacts sites which are functionally distinct from those mediated by STIM1 and Orai1. |
| doi_str_mv | 10.1016/j.cell.2013.05.026 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmedcentral</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3716012</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_3716012</sourcerecordid><originalsourceid>FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_37160123</originalsourceid><addsrcrecordid>eNqljEtLw0AURgdRbH38AVezVHTincljko0wzVzbQDMNyQh2FWKNmpKmJVHBf-8DN65dHfjOxyHkjIPDgQfXa2dVt60jgLsO-A6IYI-MOUSSeVyKfTIGiAQLA-mNyNEwrAEg9H3_kIyEKyPOZTgmXZace1f-RSaoxgyNRmOpMprGlbhkOU7v5sqippizbK6KVNEU00muDNLEWMxVbJOFKb5WnfwcJ0tqZ0jx3n7HNCuWRmV2YdU0TUxxQg6eqnaoT395TG5u0cYztnt72NSPq7p77au23PXNpuo_ym3VlH9N17yUz9v30pU8AC7cfwc-ATeiXt0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>PI(4,5)P2 DEPENDENT AND Ca2+-REGULATED ER-PLASMA MEMBRANE INTERACTIONS MEDIATED BY THE EXTENDED-SYNAPTOTAGMINS</title><source>Elsevier ScienceDirect Journals Complete</source><source>Cell Press Free Archives</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Giordano, Francesca ; Saheki, Yasunori ; Idevall-Hagren, Olof ; Colombo, Sara ; Pirruccello, Michelle ; Milosevic, Ira ; Gracheva, Elena O. ; Bagriantsev, Sviatoslav N. ; Borgese, Nica ; Camilli, Pietro De</creator><creatorcontrib>Giordano, Francesca ; Saheki, Yasunori ; Idevall-Hagren, Olof ; Colombo, Sara ; Pirruccello, Michelle ; Milosevic, Ira ; Gracheva, Elena O. ; Bagriantsev, Sviatoslav N. ; Borgese, Nica ; Camilli, Pietro De</creatorcontrib><description>Most available information on ER-plasma membrane (PM) contacts in cells of higher eukaryotes concerns proteins implicated in the regulation of Ca
2+
entry. However, growing evidence suggests that such contacts play more general roles in cell physiology, pointing to the existence of additionally ubiquitously expressed ER-PM tethers. Here we show that the three Extended-Synaptotagmins (E-Syts) are ER proteins that participate in such tethering function via C2 domain-dependent interactions with the PM that require PI(4,5)P
2
in the case of E-Syt2 and E-Syt3 and also elevation of cytosolic Ca
2+
in the case of E-Syt1. As they form heteromeric complexes, the E-Syts confer cytosolic Ca
2+
regulation to ER-PM contact formation. E-Syts-dependent contacts, however, are not required for store-operated Ca
2+
entry. Thus, the ER-PM tethering function of the E-Syts (tricalbins in yeast), mediate the formation of ER-PM contacts sites which are functionally distinct from those mediated by STIM1 and Orai1.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2013.05.026</identifier><identifier>PMID: 23791178</identifier><language>eng</language><ispartof>Cell, 2013-06, Vol.153 (7), p.1494-1509</ispartof><rights>2013 Elsevier Inc. All rights reserved 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Giordano, Francesca</creatorcontrib><creatorcontrib>Saheki, Yasunori</creatorcontrib><creatorcontrib>Idevall-Hagren, Olof</creatorcontrib><creatorcontrib>Colombo, Sara</creatorcontrib><creatorcontrib>Pirruccello, Michelle</creatorcontrib><creatorcontrib>Milosevic, Ira</creatorcontrib><creatorcontrib>Gracheva, Elena O.</creatorcontrib><creatorcontrib>Bagriantsev, Sviatoslav N.</creatorcontrib><creatorcontrib>Borgese, Nica</creatorcontrib><creatorcontrib>Camilli, Pietro De</creatorcontrib><title>PI(4,5)P2 DEPENDENT AND Ca2+-REGULATED ER-PLASMA MEMBRANE INTERACTIONS MEDIATED BY THE EXTENDED-SYNAPTOTAGMINS</title><title>Cell</title><description>Most available information on ER-plasma membrane (PM) contacts in cells of higher eukaryotes concerns proteins implicated in the regulation of Ca
2+
entry. However, growing evidence suggests that such contacts play more general roles in cell physiology, pointing to the existence of additionally ubiquitously expressed ER-PM tethers. Here we show that the three Extended-Synaptotagmins (E-Syts) are ER proteins that participate in such tethering function via C2 domain-dependent interactions with the PM that require PI(4,5)P
2
in the case of E-Syt2 and E-Syt3 and also elevation of cytosolic Ca
2+
in the case of E-Syt1. As they form heteromeric complexes, the E-Syts confer cytosolic Ca
2+
regulation to ER-PM contact formation. E-Syts-dependent contacts, however, are not required for store-operated Ca
2+
entry. Thus, the ER-PM tethering function of the E-Syts (tricalbins in yeast), mediate the formation of ER-PM contacts sites which are functionally distinct from those mediated by STIM1 and Orai1.</description><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqljEtLw0AURgdRbH38AVezVHTincljko0wzVzbQDMNyQh2FWKNmpKmJVHBf-8DN65dHfjOxyHkjIPDgQfXa2dVt60jgLsO-A6IYI-MOUSSeVyKfTIGiAQLA-mNyNEwrAEg9H3_kIyEKyPOZTgmXZace1f-RSaoxgyNRmOpMprGlbhkOU7v5sqippizbK6KVNEU00muDNLEWMxVbJOFKb5WnfwcJ0tqZ0jx3n7HNCuWRmV2YdU0TUxxQg6eqnaoT395TG5u0cYztnt72NSPq7p77au23PXNpuo_ym3VlH9N17yUz9v30pU8AC7cfwc-ATeiXt0</recordid><startdate>20130620</startdate><enddate>20130620</enddate><creator>Giordano, Francesca</creator><creator>Saheki, Yasunori</creator><creator>Idevall-Hagren, Olof</creator><creator>Colombo, Sara</creator><creator>Pirruccello, Michelle</creator><creator>Milosevic, Ira</creator><creator>Gracheva, Elena O.</creator><creator>Bagriantsev, Sviatoslav N.</creator><creator>Borgese, Nica</creator><creator>Camilli, Pietro De</creator><scope>5PM</scope></search><sort><creationdate>20130620</creationdate><title>PI(4,5)P2 DEPENDENT AND Ca2+-REGULATED ER-PLASMA MEMBRANE INTERACTIONS MEDIATED BY THE EXTENDED-SYNAPTOTAGMINS</title><author>Giordano, Francesca ; Saheki, Yasunori ; Idevall-Hagren, Olof ; Colombo, Sara ; Pirruccello, Michelle ; Milosevic, Ira ; Gracheva, Elena O. ; Bagriantsev, Sviatoslav N. ; Borgese, Nica ; Camilli, Pietro De</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_37160123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giordano, Francesca</creatorcontrib><creatorcontrib>Saheki, Yasunori</creatorcontrib><creatorcontrib>Idevall-Hagren, Olof</creatorcontrib><creatorcontrib>Colombo, Sara</creatorcontrib><creatorcontrib>Pirruccello, Michelle</creatorcontrib><creatorcontrib>Milosevic, Ira</creatorcontrib><creatorcontrib>Gracheva, Elena O.</creatorcontrib><creatorcontrib>Bagriantsev, Sviatoslav N.</creatorcontrib><creatorcontrib>Borgese, Nica</creatorcontrib><creatorcontrib>Camilli, Pietro De</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giordano, Francesca</au><au>Saheki, Yasunori</au><au>Idevall-Hagren, Olof</au><au>Colombo, Sara</au><au>Pirruccello, Michelle</au><au>Milosevic, Ira</au><au>Gracheva, Elena O.</au><au>Bagriantsev, Sviatoslav N.</au><au>Borgese, Nica</au><au>Camilli, Pietro De</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PI(4,5)P2 DEPENDENT AND Ca2+-REGULATED ER-PLASMA MEMBRANE INTERACTIONS MEDIATED BY THE EXTENDED-SYNAPTOTAGMINS</atitle><jtitle>Cell</jtitle><date>2013-06-20</date><risdate>2013</risdate><volume>153</volume><issue>7</issue><spage>1494</spage><epage>1509</epage><pages>1494-1509</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>Most available information on ER-plasma membrane (PM) contacts in cells of higher eukaryotes concerns proteins implicated in the regulation of Ca
2+
entry. However, growing evidence suggests that such contacts play more general roles in cell physiology, pointing to the existence of additionally ubiquitously expressed ER-PM tethers. Here we show that the three Extended-Synaptotagmins (E-Syts) are ER proteins that participate in such tethering function via C2 domain-dependent interactions with the PM that require PI(4,5)P
2
in the case of E-Syt2 and E-Syt3 and also elevation of cytosolic Ca
2+
in the case of E-Syt1. As they form heteromeric complexes, the E-Syts confer cytosolic Ca
2+
regulation to ER-PM contact formation. E-Syts-dependent contacts, however, are not required for store-operated Ca
2+
entry. Thus, the ER-PM tethering function of the E-Syts (tricalbins in yeast), mediate the formation of ER-PM contacts sites which are functionally distinct from those mediated by STIM1 and Orai1.</abstract><pmid>23791178</pmid><doi>10.1016/j.cell.2013.05.026</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 2013-06, Vol.153 (7), p.1494-1509 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3716012 |
| source | Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; EZB-FREE-00999 freely available EZB journals |
| title | PI(4,5)P2 DEPENDENT AND Ca2+-REGULATED ER-PLASMA MEMBRANE INTERACTIONS MEDIATED BY THE EXTENDED-SYNAPTOTAGMINS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T02%3A16%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmedcentral&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PI(4,5)P2%20DEPENDENT%20AND%20Ca2+-REGULATED%20ER-PLASMA%20MEMBRANE%20INTERACTIONS%20MEDIATED%20BY%20THE%20EXTENDED-SYNAPTOTAGMINS&rft.jtitle=Cell&rft.au=Giordano,%20Francesca&rft.date=2013-06-20&rft.volume=153&rft.issue=7&rft.spage=1494&rft.epage=1509&rft.pages=1494-1509&rft.issn=0092-8674&rft.eissn=1097-4172&rft_id=info:doi/10.1016/j.cell.2013.05.026&rft_dat=%3Cpubmedcentral%3Epubmedcentral_primary_oai_pubmedcentral_nih_gov_3716012%3C/pubmedcentral%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/23791178&rfr_iscdi=true |