The analysis of DNA adducts: The transition from 32P-postlabeling to mass spectrometry

The analysis of DNA adducts: The transition from 32P-postlabeling to mass spectrometry

•32P-postlabeling has been the method of choice for studying DNA adducts in vivo. • The lack of structural information provided by 32P-postlabeling led to hyphenation of GC, CE, and LC with MS. • HPLC or UHPLC with online column switching is approaching the sensitivity of 32P-postlabeling for DNA ad...

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Veröffentlicht in:Cancer letters 2013-06, Vol.334 (1), p.10-19
Hauptverfasser: Klaene, Joshua J., Sharma, Vaneet K., Glick, James, Vouros, Paul
Format: Artikel
Sprache:eng
Schlagworte:
32P-postlabeling
Capillary electrophoresis
DNA adduct
Gas chromatography
Liquid chromatography
Mass spectrometry
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Zusammenfassung:•32P-postlabeling has been the method of choice for studying DNA adducts in vivo. • The lack of structural information provided by 32P-postlabeling led to hyphenation of GC, CE, and LC with MS. • HPLC or UHPLC with online column switching is approaching the sensitivity of 32P-postlabeling for DNA adduct analysis. The technique of 32P-postlabeling, which was introduced in 1982 for the analysis of DNA adducts, has long been the method of choice for in vivo studies because of its high sensitivity as it requires only
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2012.08.007