Effect of micro-vibration culture system on embryo development
Purpose Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders. Materials and methods The embryonic development rates and cell numbers of blastocysts were compared between a static cu...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 2013-06, Vol.30 (6), p.835-841 |
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| container_title | Journal of assisted reproduction and genetics |
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| creator | Hur, Yong Soo Park, Jeong Hyun Ryu, Eun Kyung Park, Sung Jin Lee, Jun Ho Lee, Soo Hee Yoon, Jung Yoon, San Hyun Hur, Chang Young Lee, Won Don Lim, Jin Ho |
| description | Purpose
Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.
Materials and methods
The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (
n
= 178) and a micro-vibration culture group (
n
= 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (
n
= 159 cycles) and a micro-vibration culture group (
n
= 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.
Results
The embryonic development rate was significantly improved in the micro-vibration culture group in mice (
p
|
| doi_str_mv | 10.1007/s10815-013-0007-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3696450</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1399922248</sourcerecordid><originalsourceid>FETCH-LOGICAL-c569t-2e8b14b912fcb9545245dfd3871c602da55d3d17924a74254ad0685239ebd8ed3</originalsourceid><addsrcrecordid>eNqNkctKxDAUhoMoOo4-gBspuHETzbVNNgMyjBcQ3Og6pM2pVtpmTNqBeXszjIoKgqtczpfvJPkROqHkghJSXEZKFJWYUI5JWmOygyZUFhwXnJPdNCdSYSJydYAOY3xNjFaM76MDxnNZKKYmaLaoa6iGzNdZ11TB41VTBjs0vs-qsR3GAFlcxwG6LO1AV4a1zxysoPXLDvrhCO3Vto1w_DFO0dP14nF-i-8fbu7mV_e4krkeMANVUlFqyuqq1FJIJqSrHVcFrXLCnJXScUcLzYQtBJPCOpIrybiG0ilwfIpmW-9yLDtwVWodbGuWoelsWBtvG_Oz0jcv5tmvDM91LiRJgvMPQfBvI8TBdE2soG1tD36MhnKtNWNMqH-gBReCE7Gxnv1CX_0Y-vQThgoqlRIqxTFFdEul_40xQP11b0rMJkizDdKkIM0mSLMxn35_8NeJz-QSwLZATKX-GcK31n9a3wGRP6fp</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1415884857</pqid></control><display><type>article</type><title>Effect of micro-vibration culture system on embryo development</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Hur, Yong Soo ; Park, Jeong Hyun ; Ryu, Eun Kyung ; Park, Sung Jin ; Lee, Jun Ho ; Lee, Soo Hee ; Yoon, Jung ; Yoon, San Hyun ; Hur, Chang Young ; Lee, Won Don ; Lim, Jin Ho</creator><creatorcontrib>Hur, Yong Soo ; Park, Jeong Hyun ; Ryu, Eun Kyung ; Park, Sung Jin ; Lee, Jun Ho ; Lee, Soo Hee ; Yoon, Jung ; Yoon, San Hyun ; Hur, Chang Young ; Lee, Won Don ; Lim, Jin Ho</creatorcontrib><description>Purpose
Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.
Materials and methods
The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (
n
= 178) and a micro-vibration culture group (
n
= 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (
n
= 159 cycles) and a micro-vibration culture group (
n
= 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.
Results
The embryonic development rate was significantly improved in the micro-vibration culture group in mice (
p
< 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (
p
< 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (
p
< 0.05).
Conclusions
Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/s10815-013-0007-0</identifier><identifier>PMID: 23657828</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Adult ; Animals ; Blastocyst - cytology ; Clinical outcomes ; Culture Media ; Embryo Biology ; Embryo Culture Techniques - methods ; Embryo Implantation ; Embryo Transfer ; Embryonic Development ; Embryos ; Female ; Fertility ; Gynecology ; Human Genetics ; Humans ; Investigations ; Medicine ; Medicine & Public Health ; Mice ; Ovulation Induction ; Pregnancy ; Pregnancy Rate ; Reproductive Medicine ; Reproductive technologies ; Vibration</subject><ispartof>Journal of assisted reproduction and genetics, 2013-06, Vol.30 (6), p.835-841</ispartof><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-2e8b14b912fcb9545245dfd3871c602da55d3d17924a74254ad0685239ebd8ed3</citedby><cites>FETCH-LOGICAL-c569t-2e8b14b912fcb9545245dfd3871c602da55d3d17924a74254ad0685239ebd8ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696450/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696450/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23657828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hur, Yong Soo</creatorcontrib><creatorcontrib>Park, Jeong Hyun</creatorcontrib><creatorcontrib>Ryu, Eun Kyung</creatorcontrib><creatorcontrib>Park, Sung Jin</creatorcontrib><creatorcontrib>Lee, Jun Ho</creatorcontrib><creatorcontrib>Lee, Soo Hee</creatorcontrib><creatorcontrib>Yoon, Jung</creatorcontrib><creatorcontrib>Yoon, San Hyun</creatorcontrib><creatorcontrib>Hur, Chang Young</creatorcontrib><creatorcontrib>Lee, Won Don</creatorcontrib><creatorcontrib>Lim, Jin Ho</creatorcontrib><title>Effect of micro-vibration culture system on embryo development</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><addtitle>J Assist Reprod Genet</addtitle><description>Purpose
Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.
Materials and methods
The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (
n
= 178) and a micro-vibration culture group (
n
= 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (
n
= 159 cycles) and a micro-vibration culture group (
n
= 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.
Results
The embryonic development rate was significantly improved in the micro-vibration culture group in mice (
p
< 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (
p
< 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (
p
< 0.05).
Conclusions
Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.</description><subject>Adult</subject><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Clinical outcomes</subject><subject>Culture Media</subject><subject>Embryo Biology</subject><subject>Embryo Culture Techniques - methods</subject><subject>Embryo Implantation</subject><subject>Embryo Transfer</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>Female</subject><subject>Fertility</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Investigations</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Ovulation Induction</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Reproductive Medicine</subject><subject>Reproductive technologies</subject><subject>Vibration</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkctKxDAUhoMoOo4-gBspuHETzbVNNgMyjBcQ3Og6pM2pVtpmTNqBeXszjIoKgqtczpfvJPkROqHkghJSXEZKFJWYUI5JWmOygyZUFhwXnJPdNCdSYSJydYAOY3xNjFaM76MDxnNZKKYmaLaoa6iGzNdZ11TB41VTBjs0vs-qsR3GAFlcxwG6LO1AV4a1zxysoPXLDvrhCO3Vto1w_DFO0dP14nF-i-8fbu7mV_e4krkeMANVUlFqyuqq1FJIJqSrHVcFrXLCnJXScUcLzYQtBJPCOpIrybiG0ilwfIpmW-9yLDtwVWodbGuWoelsWBtvG_Oz0jcv5tmvDM91LiRJgvMPQfBvI8TBdE2soG1tD36MhnKtNWNMqH-gBReCE7Gxnv1CX_0Y-vQThgoqlRIqxTFFdEul_40xQP11b0rMJkizDdKkIM0mSLMxn35_8NeJz-QSwLZATKX-GcK31n9a3wGRP6fp</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Hur, Yong Soo</creator><creator>Park, Jeong Hyun</creator><creator>Ryu, Eun Kyung</creator><creator>Park, Sung Jin</creator><creator>Lee, Jun Ho</creator><creator>Lee, Soo Hee</creator><creator>Yoon, Jung</creator><creator>Yoon, San Hyun</creator><creator>Hur, Chang Young</creator><creator>Lee, Won Don</creator><creator>Lim, Jin Ho</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130601</creationdate><title>Effect of micro-vibration culture system on embryo development</title><author>Hur, Yong Soo ; Park, Jeong Hyun ; Ryu, Eun Kyung ; Park, Sung Jin ; Lee, Jun Ho ; Lee, Soo Hee ; Yoon, Jung ; Yoon, San Hyun ; Hur, Chang Young ; Lee, Won Don ; Lim, Jin Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-2e8b14b912fcb9545245dfd3871c602da55d3d17924a74254ad0685239ebd8ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Blastocyst - cytology</topic><topic>Clinical outcomes</topic><topic>Culture Media</topic><topic>Embryo Biology</topic><topic>Embryo Culture Techniques - methods</topic><topic>Embryo Implantation</topic><topic>Embryo Transfer</topic><topic>Embryonic Development</topic><topic>Embryos</topic><topic>Female</topic><topic>Fertility</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Investigations</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Ovulation Induction</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Reproductive Medicine</topic><topic>Reproductive technologies</topic><topic>Vibration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hur, Yong Soo</creatorcontrib><creatorcontrib>Park, Jeong Hyun</creatorcontrib><creatorcontrib>Ryu, Eun Kyung</creatorcontrib><creatorcontrib>Park, Sung Jin</creatorcontrib><creatorcontrib>Lee, Jun Ho</creatorcontrib><creatorcontrib>Lee, Soo Hee</creatorcontrib><creatorcontrib>Yoon, Jung</creatorcontrib><creatorcontrib>Yoon, San Hyun</creatorcontrib><creatorcontrib>Hur, Chang Young</creatorcontrib><creatorcontrib>Lee, Won Don</creatorcontrib><creatorcontrib>Lim, Jin Ho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hur, Yong Soo</au><au>Park, Jeong Hyun</au><au>Ryu, Eun Kyung</au><au>Park, Sung Jin</au><au>Lee, Jun Ho</au><au>Lee, Soo Hee</au><au>Yoon, Jung</au><au>Yoon, San Hyun</au><au>Hur, Chang Young</au><au>Lee, Won Don</au><au>Lim, Jin Ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of micro-vibration culture system on embryo development</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><stitle>J Assist Reprod Genet</stitle><addtitle>J Assist Reprod Genet</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>30</volume><issue>6</issue><spage>835</spage><epage>841</epage><pages>835-841</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><abstract>Purpose
Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.
Materials and methods
The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (
n
= 178) and a micro-vibration culture group (
n
= 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (
n
= 159 cycles) and a micro-vibration culture group (
n
= 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.
Results
The embryonic development rate was significantly improved in the micro-vibration culture group in mice (
p
< 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (
p
< 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (
p
< 0.05).
Conclusions
Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23657828</pmid><doi>10.1007/s10815-013-0007-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1058-0468 |
| ispartof | Journal of assisted reproduction and genetics, 2013-06, Vol.30 (6), p.835-841 |
| issn | 1058-0468 1573-7330 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3696450 |
| source | MEDLINE; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adult Animals Blastocyst - cytology Clinical outcomes Culture Media Embryo Biology Embryo Culture Techniques - methods Embryo Implantation Embryo Transfer Embryonic Development Embryos Female Fertility Gynecology Human Genetics Humans Investigations Medicine Medicine & Public Health Mice Ovulation Induction Pregnancy Pregnancy Rate Reproductive Medicine Reproductive technologies Vibration |
| title | Effect of micro-vibration culture system on embryo development |
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