Transcriptional insights into the CD8+ T cell response to infection and memory T cell formation

Antigenic activation induces the generation of effector and memory CD8 + T cells. Goldrath and colleagues profile gene expression at various times after infection to identify transcriptional networks unique to each population. After infection, many factors coordinate the population expansion and differentiation of CD8 + effector and memory T cells. Using data of unparalleled breadth from the Immunological Genome Project, we analyzed the CD8 + T cell transcriptome throughout infection to establish gene-expression signatures and identify putative transcriptional regulators. Notably, we found that the expression of key gene signatures can be used to predict the memory-precursor potential of CD8 + effector cells. Long-lived memory CD8 + cells ultimately expressed a small subset of genes shared by natural killer T and γδ T cells. Although distinct inflammatory milieu and T cell precursor frequencies influenced the differentiation of CD8 + effector and memory populations, core transcriptional signatures were regulated similarly, whether polyclonal or transgenic, and whether responding to bacterial or viral model pathogens. Our results provide insights into the transcriptional regulation that influence memory formation and CD8 + T cell immunity.