Bioactive Surface Modification of Hydroxyapatite
The purpose of this study was to establish an acid-etching procedure for altering the Ca/P ratio of the nanostructured surface of hydroxyapatite (HAP) by using surface chemical and morphological analyses (XPS, XRD, SEM, surface roughness, and wettability) and to evaluate the in vitro response of ost...
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| Veröffentlicht in: | BioMed research international 2013-01, Vol.2013 (2013), p.1-9 |
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| creator | Hirata, Isao Nogami, Keisuke Yasuda, Keisuke Hiasa, Kyou Okazaki, Yohei Abe, Yasuhiko Mizumachi, Wataru |
| description | The purpose of this study was to establish an acid-etching procedure for altering the Ca/P ratio of the nanostructured surface of hydroxyapatite (HAP) by using surface chemical and morphological analyses (XPS, XRD, SEM, surface roughness, and wettability) and to evaluate the in vitro response of osteoblast-like cells (MC3T3-E1 cells) to the modified surfaces. This study utilized HAP and HAP treated with 10%, 20%, 30%, 40%, 50%, or 60% phosphoric acid solution for 10 minutes at 25°C, followed by rinsing 3 times with ultrapure water. The 30% phosphoric acid etching process that provided a Ca/P ratio of 1.50, without destruction of the grain boundary of HAP, was selected as a surface-modification procedure. Additionally, HAP treated by the 30% phosphoric acid etching process was stored under dry conditions at 25°C for 12 hours, and the Ca/P ratio approximated to 1.00 accidentally. The initial adhesion, proliferation, and differentiation (alkaline phosphatase (ALP) activity and relative mRNA level for ALP) of MC3T3-E1 cells on the modified surfaces were significantly promoted (P |
| doi_str_mv | 10.1155/2013/626452 |
| format | Article |
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This study utilized HAP and HAP treated with 10%, 20%, 30%, 40%, 50%, or 60% phosphoric acid solution for 10 minutes at 25°C, followed by rinsing 3 times with ultrapure water. The 30% phosphoric acid etching process that provided a Ca/P ratio of 1.50, without destruction of the grain boundary of HAP, was selected as a surface-modification procedure. Additionally, HAP treated by the 30% phosphoric acid etching process was stored under dry conditions at 25°C for 12 hours, and the Ca/P ratio approximated to 1.00 accidentally. The initial adhesion, proliferation, and differentiation (alkaline phosphatase (ALP) activity and relative mRNA level for ALP) of MC3T3-E1 cells on the modified surfaces were significantly promoted (P<0.05 and 0.01). These findings show that the 30% phosphoric acid etching process for the nanostructured HAP surface can alter the Ca/P ratio effectively and may accelerate the initial adhesion, proliferation, and differentiation of MC3T3-E1 cells.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2013/626452</identifier><identifier>PMID: 23862150</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; Analysis ; Animals ; Biocompatible Materials - pharmacology ; Cell Adhesion - drug effects ; Cell Differentiation - drug effects ; Cell Line ; Cell Proliferation - drug effects ; Durapatite - pharmacology ; Mice ; Microscopy, Electron, Scanning ; Nanotechnology ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - enzymology ; Phosphoric acid ; Photoelectron Spectroscopy ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Thermodynamics ; Wettability - drug effects ; X-Ray Diffraction</subject><ispartof>BioMed research international, 2013-01, Vol.2013 (2013), p.1-9</ispartof><rights>Copyright © 2013 Yasuhiko Abe et al.</rights><rights>COPYRIGHT 2013 John Wiley & Sons, Inc.</rights><rights>Copyright © 2013 Yasuhiko Abe et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-7c2c70c07db8d0851de28e5b422fa9579cb98844350d36bbdf80595a4fbdedcb3</citedby><cites>FETCH-LOGICAL-c533t-7c2c70c07db8d0851de28e5b422fa9579cb98844350d36bbdf80595a4fbdedcb3</cites><orcidid>0000-0002-5306-5073</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687726/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687726/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23862150$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sauro, Salvatore</contributor><creatorcontrib>Hirata, Isao</creatorcontrib><creatorcontrib>Nogami, Keisuke</creatorcontrib><creatorcontrib>Yasuda, Keisuke</creatorcontrib><creatorcontrib>Hiasa, Kyou</creatorcontrib><creatorcontrib>Okazaki, Yohei</creatorcontrib><creatorcontrib>Abe, Yasuhiko</creatorcontrib><creatorcontrib>Mizumachi, Wataru</creatorcontrib><title>Bioactive Surface Modification of Hydroxyapatite</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>The purpose of this study was to establish an acid-etching procedure for altering the Ca/P ratio of the nanostructured surface of hydroxyapatite (HAP) by using surface chemical and morphological analyses (XPS, XRD, SEM, surface roughness, and wettability) and to evaluate the in vitro response of osteoblast-like cells (MC3T3-E1 cells) to the modified surfaces. This study utilized HAP and HAP treated with 10%, 20%, 30%, 40%, 50%, or 60% phosphoric acid solution for 10 minutes at 25°C, followed by rinsing 3 times with ultrapure water. The 30% phosphoric acid etching process that provided a Ca/P ratio of 1.50, without destruction of the grain boundary of HAP, was selected as a surface-modification procedure. Additionally, HAP treated by the 30% phosphoric acid etching process was stored under dry conditions at 25°C for 12 hours, and the Ca/P ratio approximated to 1.00 accidentally. The initial adhesion, proliferation, and differentiation (alkaline phosphatase (ALP) activity and relative mRNA level for ALP) of MC3T3-E1 cells on the modified surfaces were significantly promoted (P<0.05 and 0.01). These findings show that the 30% phosphoric acid etching process for the nanostructured HAP surface can alter the Ca/P ratio effectively and may accelerate the initial adhesion, proliferation, and differentiation of MC3T3-E1 cells.</description><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Analysis</subject><subject>Animals</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Durapatite - pharmacology</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nanotechnology</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - enzymology</subject><subject>Phosphoric acid</subject><subject>Photoelectron Spectroscopy</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Thermodynamics</subject><subject>Wettability - drug effects</subject><subject>X-Ray Diffraction</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc1LwzAchoMobsydvEvBiyh1-WjS9CLMoU6YeFDPIc3HFuma2Y_p_nszOofeDIGEXx4eXvICcIrgNUKUjjBEZMQwSyg-AH1MUBIzlKDD_Z2QHhjW9TsMiyMGM3YMephwhhGFfQBvnZeqcWsTvbSVlcpET14765RsnC8jb6PpRlf-ayNXYdKYE3BkZVGb4e4cgLf7u9fJNJ49PzxOxrNYUUKaOFVYpVDBVOdcQ06RNpgbmicYW5nRNFN5xnmSEAo1YXmuLYc0ozKxuTZa5WQAbjrvqs2XYWLKppKFWFVuKauN8NKJvy-lW4i5XwvCeJpiFgQXO0HlP1pTN2LpamWKQpbGt7VACUQwQ5BlAT3v0LksjHCl9cGotrgYk5SEjTISqKuOUpWv68rYfRgExbYMsS1DdGUE-ux3_j378_UBuOyAhSu1_HT_s5mAGCt_wQSGeOQb-FeZ5w</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Hirata, Isao</creator><creator>Nogami, Keisuke</creator><creator>Yasuda, Keisuke</creator><creator>Hiasa, Kyou</creator><creator>Okazaki, Yohei</creator><creator>Abe, Yasuhiko</creator><creator>Mizumachi, Wataru</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5306-5073</orcidid></search><sort><creationdate>20130101</creationdate><title>Bioactive Surface Modification of Hydroxyapatite</title><author>Hirata, Isao ; Nogami, Keisuke ; Yasuda, Keisuke ; Hiasa, Kyou ; Okazaki, Yohei ; Abe, Yasuhiko ; Mizumachi, Wataru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-7c2c70c07db8d0851de28e5b422fa9579cb98844350d36bbdf80595a4fbdedcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alkaline Phosphatase - genetics</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Analysis</topic><topic>Animals</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Durapatite - pharmacology</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nanotechnology</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - enzymology</topic><topic>Phosphoric acid</topic><topic>Photoelectron Spectroscopy</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Thermodynamics</topic><topic>Wettability - drug effects</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hirata, Isao</creatorcontrib><creatorcontrib>Nogami, Keisuke</creatorcontrib><creatorcontrib>Yasuda, Keisuke</creatorcontrib><creatorcontrib>Hiasa, Kyou</creatorcontrib><creatorcontrib>Okazaki, Yohei</creatorcontrib><creatorcontrib>Abe, Yasuhiko</creatorcontrib><creatorcontrib>Mizumachi, Wataru</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hirata, Isao</au><au>Nogami, Keisuke</au><au>Yasuda, Keisuke</au><au>Hiasa, Kyou</au><au>Okazaki, Yohei</au><au>Abe, Yasuhiko</au><au>Mizumachi, Wataru</au><au>Sauro, Salvatore</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioactive Surface Modification of Hydroxyapatite</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>The purpose of this study was to establish an acid-etching procedure for altering the Ca/P ratio of the nanostructured surface of hydroxyapatite (HAP) by using surface chemical and morphological analyses (XPS, XRD, SEM, surface roughness, and wettability) and to evaluate the in vitro response of osteoblast-like cells (MC3T3-E1 cells) to the modified surfaces. This study utilized HAP and HAP treated with 10%, 20%, 30%, 40%, 50%, or 60% phosphoric acid solution for 10 minutes at 25°C, followed by rinsing 3 times with ultrapure water. The 30% phosphoric acid etching process that provided a Ca/P ratio of 1.50, without destruction of the grain boundary of HAP, was selected as a surface-modification procedure. Additionally, HAP treated by the 30% phosphoric acid etching process was stored under dry conditions at 25°C for 12 hours, and the Ca/P ratio approximated to 1.00 accidentally. The initial adhesion, proliferation, and differentiation (alkaline phosphatase (ALP) activity and relative mRNA level for ALP) of MC3T3-E1 cells on the modified surfaces were significantly promoted (P<0.05 and 0.01). These findings show that the 30% phosphoric acid etching process for the nanostructured HAP surface can alter the Ca/P ratio effectively and may accelerate the initial adhesion, proliferation, and differentiation of MC3T3-E1 cells.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>23862150</pmid><doi>10.1155/2013/626452</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5306-5073</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Analysis Animals Biocompatible Materials - pharmacology Cell Adhesion - drug effects Cell Differentiation - drug effects Cell Line Cell Proliferation - drug effects Durapatite - pharmacology Mice Microscopy, Electron, Scanning Nanotechnology Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - enzymology Phosphoric acid Photoelectron Spectroscopy RNA, Messenger - genetics RNA, Messenger - metabolism Thermodynamics Wettability - drug effects X-Ray Diffraction |
| title | Bioactive Surface Modification of Hydroxyapatite |
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