Back-Scattering Interferometry: An Ultrasensitive Method for the Unperturbed Detection of Acetylcholinesterase-Inhibitor Interactions

A series of inhibitors of acetylcholinesterase (AChE) have been screened by back‐scattering interferometry (BSI). Enzyme levels as low as 100 pM (22 000 molecules of AChE) can be detected. This method can be used to screen for mixed AChE inhibitors, agents that have shown high efficacy against Alzhe...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Angewandte Chemie International Edition 2012-10, Vol.51 (44), p.11126-11130
Hauptverfasser:	Haddad, Gabrielle L., Young, Sherri C., Heindel, Ned D., Bornhop, Darryl J., Flowers II, Robert A.
Format:	Artikel
Sprache:	eng
Schlagworte:	acetylcholinesterase Acetylcholinesterase - chemistry Acetylcholinesterase - metabolism Alzheimer's disease analytical methods Animals Cholinesterase Inhibitors - chemistry Cholinesterase Inhibitors - pharmacology Effectiveness Electrophorus enzyme assays Enzymes high-throughput screening Inhibitors Interferometry Level (quantity) Light Molecular Structure Screens
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A series of inhibitors of acetylcholinesterase (AChE) have been screened by back‐scattering interferometry (BSI). Enzyme levels as low as 100 pM (22 000 molecules of AChE) can be detected. This method can be used to screen for mixed AChE inhibitors, agents that have shown high efficacy against Alzheimer's disease, by detecting dual‐binding interactions. E=enzyme, I=inhibitor, S=substrate.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201203640