Functional evaluation of malaria Pfs25 DNA vaccine by in vivo electroporation in olive baboons

Highlights • We evaluated DNA vaccine encoding Pfs25, a malaria vaccine candidate antigen. • DNA delivery by in vivo electroporation in baboons elicited functional antibodies. • DNA prime and protein boost further enhanced blocking antibody response. • We also validated Pfs25TrPb-murine model for fu...

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Veröffentlicht in:	Vaccine 2013-06, Vol.31 (31), p.3140-3147
Hauptverfasser:	Kumar, Rajesh, Nyakundi, Ruth, Kariuki, Thomas, Ozwara, Hastings, Nyamongo, Onkoba, Mlambo, Godfree, Ellefsen, Barry, Hannaman, Drew, Kumar, Nirbhay
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology animal models Animals Antibodies, Protozoan - blood Antibody Affinity Antibody Formation antigens Antigens, Protozoan - genetics Antigens, Protozoan - immunology Applied microbiology Biological and medical sciences DNA vaccine Dose-Response Relationship, Immunologic Electroporation Female Fundamental and applied biological sciences. Psychology Human protozoal diseases humans immune response Immunization, Secondary Infectious diseases Malaria Malaria Vaccines - administration & dosage Malaria Vaccines - genetics Malaria Vaccines - immunology Malaria, Falciparum - immunology Malaria, Falciparum - prevention & control Medical sciences Mice Microbiology Olea ookinetes Papio Papio anubis Papio anubis - immunology Parasitic diseases Pfs25 Plasmids Plasmodium falciparum Primates Prime boost Protozoal diseases Protozoan Proteins - genetics Protozoan Proteins - immunology Recombinant Proteins - genetics Recombinant Proteins - immunology recombinant vaccines Transmission blocking vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology volunteers zygote
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Beschreibung
Zusammenfassung:	Highlights • We evaluated DNA vaccine encoding Pfs25, a malaria vaccine candidate antigen. • DNA delivery by in vivo electroporation in baboons elicited functional antibodies. • DNA prime and protein boost further enhanced blocking antibody response. • We also validated Pfs25TrPb-murine model for future evaluation of antibodies.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2013.05.006