Cell labeling approaches for fluorescence‐based in vivo flow cytometry

We provide an overview of the methods used to label circulating cells for fluorescence detection by in vivo flow cytometry. These methods are useful for cell tracking in small animals without the need to draw blood samples and are particularly useful for the detection of circulating cancer cells and...

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Veröffentlicht in:	Cytometry. Part A 2011-10, Vol.79A (10), p.758-765
Hauptverfasser:	Pitsillides, Costas M., Runnels, Judith M., Spencer, Joel A., Zhi, Liang, Wu, Mei X., Lin, Charles P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adoptive Transfer Animals Antibodies - analysis Antibodies - metabolism Cancer Cell Line, Tumor cell tracking circulating immune cells circulating tumor cells Flow cytometry Flow Cytometry - methods Fluorescence Fluorescent Dyes - analysis Fluorescent Dyes - chemistry Green Fluorescent Proteins - analysis Green Fluorescent Proteins - chemistry Humans in vivo flow cytometry Mice Molecular Imaging - methods Neoplasms - diagnosis Neoplasms - pathology Neoplastic Cells, Circulating - pathology Photoacoustic Techniques - methods Reviews Staining and Labeling - methods
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Beschreibung
Zusammenfassung:	We provide an overview of the methods used to label circulating cells for fluorescence detection by in vivo flow cytometry. These methods are useful for cell tracking in small animals without the need to draw blood samples and are particularly useful for the detection of circulating cancer cells and quantification of circulating immune cells. © 2011 International Society for Advancement of Cytometry.
ISSN:	1552-4922 1552-4930 1552-4930
DOI:	10.1002/cyto.a.21125