Solid-phase synthesis of C-terminal peptide libraries for studying the specificity of enzymatic protein prenylation

Solid-phase synthesis of C-terminal peptide libraries for studying the specificity of enzymatic protein prenylation

Prenylation is an essential post-translational modification in all eukaryotes. Here we describe the synthesis of a 340-member library of peptides containing free C-termini on cellulose membranes. The resulting library was then used to probe the specificity of protein farnesyltransferase from S. cere...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2012-01, Vol.48 (66), p.8228-8230
Hauptverfasser: Wang, Yen-Chih, Distefano, Mark D
Format: Artikel
Sprache:eng
Schlagworte:
Farnesyltranstransferase - chemistry
Molecular Structure
Peptide Library
Peptides - chemical synthesis
Protein Prenylation
Saccharomyces cerevisiae - enzymology
Solid-Phase Synthesis Techniques
Substrate Specificity
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Prenylation is an essential post-translational modification in all eukaryotes. Here we describe the synthesis of a 340-member library of peptides containing free C-termini on cellulose membranes. The resulting library was then used to probe the specificity of protein farnesyltransferase from S. cerevisiae.
ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc31713c