Adaptive optics enables 3D STED microscopy in aberrating specimens

Adaptive optics enables 3D STED microscopy in aberrating specimens

Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implem...

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Veröffentlicht in:Optics express 2012-09, Vol.20 (19), p.20998-21009
Hauptverfasser: Gould, Travis J, Burke, Daniel, Bewersdorf, Joerg, Booth, Martin J
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Microscopy, Fluorescence - methods
Microspheres
Optical Phenomena
Optics and Photonics - methods
Retina - anatomy & histology
Zebrafish
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Zusammenfassung:Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.20.020998