Influence of limonin on Wnt signalling molecule in HepG2 cell lines

Influence of limonin on Wnt signalling molecule in HepG2 cell lines

Objective: The role of limonin as potent anti carcinogenic, apoptosis and chemotherapeutic agents has been supported by limited studies. Materials and Methods: In this study, limonin is identified as a potent anti proliferative agent against human hepatoma HepG2 cells based on the cell viability stu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of natural science, biology and medicine biology and medicine, 2013-01, Vol.4 (1), p.126-133
Hauptverfasser: Langeswaran, K, Gowthamkumar, S, Vijayaprakash, S, Revathy, R, Balasubramanian, M
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Cancer therapies
Cell culture
Cell division
Dehydrogenases
Gene expression
Hepatitis
Kinases
Laboratories
Liver cancer
Liver cirrhosis
Medical prognosis
Morphology
Mutation
Original
Protein expression
Proteins
Studies
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Objective: The role of limonin as potent anti carcinogenic, apoptosis and chemotherapeutic agents has been supported by limited studies. Materials and Methods: In this study, limonin is identified as a potent anti proliferative agent against human hepatoma HepG2 cells based on the cell viability study, LDH leakage assay. Induction of apoptosis in HepG2 cells by limonin was evidenced by western blot analysis of Bax, Cyclin D1, Caspase 3 and Caspase9. Results: Since Wnt signalling is involved in the initiation and sustaining of hepatocellular carcinoma we studied differential expression of LRP5, LRP6 and DKK wnt players. Conclusion: Limonin found to down regulate these players which forms a rationale for further investigation on effect on limonin in cancer therapy.
ISSN:0976-9668
2229-7707
DOI:10.4103/0976-9668.107276