Antiproliferative role of Indigofera aspalathoides on 20 methylcholanthrene induced fibrosarcoma in rats

Objective:To find out the anticancer effect of indigofera aspalathoides(L.aspalathoides)on20-methylcholanthrene induced fibrosarcoma in rats.Methods:Fibrosarcoma was induced in Wistar strain male albino rats by 2O-methylcholanthrene.Intraperitoneous(i.p.)administration of250 mg/kg body weight/day of aqueous extract of I.aspalathoides for 30 d effectively suppressed chemically induced tumors.Parameters such as body weight,liver and kidney weight,tumor weight,mean survival time,behavioral changes,blood glucose,blood glycogen and marker enzymes such as alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),acid phosphatase(ACP)and 5’-nucleiotidase(5’-NT)in serum,liver and kidney and lipid profiles such as total cholesterel,phospholipids,free fatty acids in liver and kidney of control and experimental animals were studied.Results:Fibrosarcoma bearing animals were ferocious and anxious.The mean survival time was found to increase after the treatment.The body weights were significantly decreased(P<0.001)in groupⅡfibrosarcoma animals which steadily increased after the treatment with I.aspalathoides.The liver and kidney weights were significantly increased whereas the tumor weights decreased as compared to the weights in untreated fibrosarcoma bearing rats.The blood glucose and the liver and kidney glycogen levels were found to decrease significantly(P<0.001)in groupⅡanimals.Elevated activities of marker enzymes were observed in serum,liver and kidney of fibrosarcoma bearing GroupⅡanimals which were normalize after I.aspalathoides treatment.In the liver and kidney of GroupⅡanimals the total cholesterol increased whereas the pbospbolipids and free fatty acid levels decreased(P<0.001)which were normalized after treatment.Conclusions:The treatment by I.aspalathoides on fihrosarcoma bearing rats has improved the levels of various parameters indicating its antiproliferative and anticancer activity.