Gamma-Tocotrienol Modulated Gene Expression in Senescent Human Diploid Fibroblasts as Revealed by Microarray Analysis

The effect of γ-tocotrienol, a vitamin E isomer, in modulating gene expression in cellular aging of human diploid fibroblasts was studied. Senescent cells at passage 30 were incubated with 70 μM of γ-tocotrienol for 24 h. Gene expression patterns were evaluated using Sentrix HumanRef-8 Expression Be...

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Veröffentlicht in:	Oxidative medicine and cellular longevity 2013-01, Vol.2013 (2013), p.1-11
Hauptverfasser:	Makpol, Suzana, Zainuddin, Azalina, Kien Hui, Chua, Mohd Yusof, Yasmin Anum, Ngah, Wan Zurinah Wan
Format:	Artikel
Sprache:	eng
Schlagworte:	Cells, Cultured Cellular Senescence - drug effects Chromans - pharmacology Cluster Analysis Diploidy Fibroblasts - drug effects Fibroblasts - metabolism Gene Expression Regulation - drug effects Gene Regulatory Networks Humans Oligonucleotide Array Sequence Analysis Real-Time Polymerase Chain Reaction Vitamin E - analogs & derivatives Vitamin E - pharmacology
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Zusammenfassung:	The effect of γ-tocotrienol, a vitamin E isomer, in modulating gene expression in cellular aging of human diploid fibroblasts was studied. Senescent cells at passage 30 were incubated with 70 μM of γ-tocotrienol for 24 h. Gene expression patterns were evaluated using Sentrix HumanRef-8 Expression BeadChip from Illumina, analysed using GeneSpring GX10 software, and validated using quantitative RT-PCR. A total of 100 genes were differentially expressed (P
ISSN:	1942-0900 1942-0994
DOI:	10.1155/2013/454328