Antioxidant properties and inhibitory effects of Satureja khozestanica essential oil on LDL oxidation induced-CuSO4 in vitro

Objective:To assess various antioxidative activities of Satureja khozestanica essential oil（SKE）and its effect on oxidation of low density lipoprotein（LDL）induced by CuSO4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde（MDA）.Methods:The formation of conjugated dienes,lag time and MDA were measured.Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE.Also total antioxidant activity and free radical scavenging of SKE were determinated.Results:It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation.The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200μg/mL.Total antioxidant capacity of SKE was（3.20±0.40）nmol of ascorbic acid equivalents/g SKE.The SKE showed remarkable scavenging activity on 2,2-diphenyl-picrylhydrazyl,IC50（5.30±0.11）ng/mL.Conclusions:This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications.