Anti-dermatophytic activity of marine sponge,Sigmadocia carnosa(Dendy)on clinically isolated fungi

Objective:To screen the anti-fungal effects and find out the active metabolites from sponge, Sigmadocia carnosa(S.carnosa) against four dermatophytic fungi.Methods:The methanol,ethyl acetate and acetone extract of marine sponge,S.carnosa was examined against Trichaphyton mentagrophytes(T.mentagrophytes),Trichophyton rubrum(T.rubrum),Epidermophyton floccosum(E.floccosum) and Microsporum gypseum(M.gypseum) and qualitative analysed to find out the active molecules.Results:The methanol extract of sponge was expressed significant activity than ethyl acetate and acetone.The minimum inhibitory concentration(MIC) of methanol extract of sponge that resulted in complete growth inhibition of T.mentagrophytes,T.rubrum, E.floccosum and M.gypseum were found to 125,250,250 and 250 μg/mL respectively.But, 100%inhibition of fungal spore germination was observed in T.mentagrophytes at 500 μg/mL concentration followed by T.rubrum,E.floccosum and At.gypseum at 1000 μg/mL concentration. Other two extracts showed weak anti spore germination activity against the tested dermatophyte fungi.Methanol extracts showed presence of terpenoids,steroids,alkaloids,saponins and glycosides.Conclusion:Based on the literature,this is the first study which has conducted to inhibit the growth and spore germination of dermatophytic fungi with S.carnosa.Further research also needs to purify and characterize the secondary metabolites from the sponge,5.carnosa for the valuable source of novel substances for future drug discovery.