Lewis acid catalysis of phosphoryl transfer from a copper(II)-NTP complex in a kinase ribozyme

The chemical strategies used by ribozymes to enhance reaction rates are revealed in part from their metal ion and pH requirements. We find that kinase ribozyme K28(1-77)C, in contrast with previously characterized kinase ribozymes, requires Cu(2+) for optimal catalysis of thiophosphoryl transfer fro...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 2013-03, Vol.41 (5), p.3327-3338
Hauptverfasser:	Biondi, Elisa, Poudyal, Raghav R, Forgy, Joshua C, Sawyer, Andrew W, Maxwell, Adam W R, Burke, Donald H
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - chemistry Base Sequence Buffers Catalysis Catalytic Domain Coordination Complexes - chemistry Copper - chemistry Hydrogen-Ion Concentration Lewis Acids Magnesium - chemistry Nucleic Acid Conformation Nucleic Acid Enzymes Phosphorylation Phosphotransferases - chemistry RNA, Catalytic - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!