Killing Effect of Ad5/F35-APE1 siRNA Recombinant Adenovirus in Combination with Hematoporphrphyrin Derivative-Mediated Photodynamic Therapy on Human Nonsmall Cell Lung Cancer

The main goal of this work is to investigate the killing effects and molecular mechanism of photodynamic therapy (PDT) mediated by the Ad5/F35-APE1 siRNA recombinant adenovirus in combination with a hematoporphrphyrin derivative (HpD) in the A549 human lung adenocarcinoma cell line in vitro to provi...

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Veröffentlicht in:	BioMed research international 2013-01, Vol.2013 (2013), p.1-7
Hauptverfasser:	Yang, Zhen-Zhou, Li, Zeng-Peng, Zeng, Lin-Li, Zhang, Yun-Song, Wang, Dong, Guan, Wei, Xia, Lei, Wang, Ge
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma Adenoviridae - genetics Adenovirus Apoptosis Carcinoma, Non-Small-Cell Lung - pathology Carcinoma, Non-Small-Cell Lung - therapy Cell Line, Tumor DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics Enzyme-Linked Immunosorbent Assay Flow Cytometry Genetic Therapy - methods Genetic Vectors Hematoporphyrins - pharmacology Humans Inflammation Lung Neoplasms - pathology Lung Neoplasms - therapy Photochemotherapy - methods Reactive Oxygen Species RNA, Small Interfering - metabolism
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Zusammenfassung:	The main goal of this work is to investigate the killing effects and molecular mechanism of photodynamic therapy (PDT) mediated by the Ad5/F35-APE1 siRNA recombinant adenovirus in combination with a hematoporphrphyrin derivative (HpD) in the A549 human lung adenocarcinoma cell line in vitro to provide a theoretical reference for treating lung cancer by HpD-PDT. By using the technologies of MTT, flow cytometry, ELISA, and western blot, we observed that the proliferation inhibition and apoptosis of the A549 cells were significantly higher than the control group (P
ISSN:	2314-6133 2314-6141
DOI:	10.1155/2013/957913