Rhamnogalacturonan II is a Toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells

We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype...

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Veröffentlicht in:Experimental & molecular medicine 2013-02, Vol.45 (2), p.e8-e8
Hauptverfasser: Park, Sung Nam, Tae Noh, Kyung, Jeong, Young-Il, Duk Jung, In, Kyu Kang, Hyun, Sun Cha, Gil, Jung Lee, Su, Keun Seo, Jong, Hwan Kang, Dae, Hwang, Tae-Ho, Kyung Lee, Eun, Kwon, Byungsuk, Park, Yeong-Min
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container_title Experimental & molecular medicine
container_volume 45
creator Park, Sung Nam
Tae Noh, Kyung
Jeong, Young-Il
Duk Jung, In
Kyu Kang, Hyun
Sun Cha, Gil
Jung Lee, Su
Keun Seo, Jong
Hwan Kang, Dae
Hwang, Tae-Ho
Kyung Lee, Eun
Kwon, Byungsuk
Park, Yeong-Min
description We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1β, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8 + T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway. Cancer immunotherapy: Ginseng extract yields potent adjuvant Researchers in Korea report that the immune response against cancer is enhanced by a complex sugar found in the walls of plant cells. Led by Yeong-Min Park of the Pusan National University in Yangsan, the researchers purified the sugar, a polysaccharide called rhamnogalacturonan-II (RG-II), from the leaves of the Asian ginseng plant. In cell culture, the researchers added RG-II to a type of immune cell – dendritic cells – derived from the bone marrow of mice. They showed that the sugar triggered the production of specialized T cells capable of destroying cancerous tissue. They then injected RG-II–treated dendritic cells below the skin of mice with lymphoma and discovered that the treatment delayed tumor growth in the animals. The authors propose that RG-II could be a safe and effective adjuvant to enhance the tumor-killing potential of dendritic cell–based vaccines.
doi_str_mv 10.1038/emm.2013.14
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BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1β, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8 + T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway. Cancer immunotherapy: Ginseng extract yields potent adjuvant Researchers in Korea report that the immune response against cancer is enhanced by a complex sugar found in the walls of plant cells. Led by Yeong-Min Park of the Pusan National University in Yangsan, the researchers purified the sugar, a polysaccharide called rhamnogalacturonan-II (RG-II), from the leaves of the Asian ginseng plant. 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Tae Noh, Kyung ; Jeong, Young-Il ; Duk Jung, In ; Kyu Kang, Hyun ; Sun Cha, Gil ; Jung Lee, Su ; Keun Seo, Jong ; Hwan Kang, Dae ; Hwang, Tae-Ho ; Kyung Lee, Eun ; Kwon, Byungsuk ; Park, Yeong-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-79349f3c4f7e509990501f7a224233c78605f2b1f2fb785419aad180c0b25ef23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acute-Phase Proteins - metabolism</topic><topic>Adaptor Proteins, Vesicular Transport - metabolism</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Carrier Proteins - metabolism</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytokines - biosynthesis</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - enzymology</topic><topic>Dendritic Cells - immunology</topic><topic>Enzyme Activation - drug effects</topic><topic>Lipopolysaccharide Receptors - metabolism</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Medical Biochemistry</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Molecular Medicine</topic><topic>Myeloid Differentiation Factor 88 - metabolism</topic><topic>Neoplasms - immunology</topic><topic>Neoplasms - pathology</topic><topic>NF-kappa B - metabolism</topic><topic>Original</topic><topic>original-article</topic><topic>Pectins - pharmacology</topic><topic>Phenotype</topic><topic>Protein Transport - drug effects</topic><topic>Receptors, Chemokine - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Stem Cells</topic><topic>T-Lymphocytes, Cytotoxic - cytology</topic><topic>T-Lymphocytes, Cytotoxic - drug effects</topic><topic>Toll-Like Receptor 4 - agonists</topic><topic>Toll-Like Receptor 4 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Sung Nam</creatorcontrib><creatorcontrib>Tae Noh, Kyung</creatorcontrib><creatorcontrib>Jeong, Young-Il</creatorcontrib><creatorcontrib>Duk Jung, In</creatorcontrib><creatorcontrib>Kyu Kang, Hyun</creatorcontrib><creatorcontrib>Sun Cha, Gil</creatorcontrib><creatorcontrib>Jung Lee, Su</creatorcontrib><creatorcontrib>Keun Seo, Jong</creatorcontrib><creatorcontrib>Hwan Kang, Dae</creatorcontrib><creatorcontrib>Hwang, Tae-Ho</creatorcontrib><creatorcontrib>Kyung Lee, Eun</creatorcontrib><creatorcontrib>Kwon, Byungsuk</creatorcontrib><creatorcontrib>Park, Yeong-Min</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; 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molecular medicine</jtitle><stitle>Exp Mol Med</stitle><addtitle>Exp Mol Med</addtitle><date>2013-02-08</date><risdate>2013</risdate><volume>45</volume><issue>2</issue><spage>e8</spage><epage>e8</epage><pages>e8-e8</pages><issn>2092-6413</issn><issn>1226-3613</issn><eissn>2092-6413</eissn><abstract>We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1β, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8 + T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway. Cancer immunotherapy: Ginseng extract yields potent adjuvant Researchers in Korea report that the immune response against cancer is enhanced by a complex sugar found in the walls of plant cells. Led by Yeong-Min Park of the Pusan National University in Yangsan, the researchers purified the sugar, a polysaccharide called rhamnogalacturonan-II (RG-II), from the leaves of the Asian ginseng plant. In cell culture, the researchers added RG-II to a type of immune cell – dendritic cells – derived from the bone marrow of mice. They showed that the sugar triggered the production of specialized T cells capable of destroying cancerous tissue. They then injected RG-II–treated dendritic cells below the skin of mice with lymphoma and discovered that the treatment delayed tumor growth in the animals. The authors propose that RG-II could be a safe and effective adjuvant to enhance the tumor-killing potential of dendritic cell–based vaccines.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23392255</pmid><doi>10.1038/emm.2013.14</doi><oa>free_for_read</oa></addata></record>
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subjects Acute-Phase Proteins - metabolism
Adaptor Proteins, Vesicular Transport - metabolism
Animals
Biomedical and Life Sciences
Biomedicine
Bone Marrow Cells - cytology
Bone Marrow Cells - drug effects
Carrier Proteins - metabolism
CD8-Positive T-Lymphocytes - immunology
Cell Differentiation - drug effects
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cell Proliferation - drug effects
Cytokines - biosynthesis
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - enzymology
Dendritic Cells - immunology
Enzyme Activation - drug effects
Lipopolysaccharide Receptors - metabolism
Lymphocyte Activation - drug effects
Medical Biochemistry
Membrane Glycoproteins - metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Mitogen-Activated Protein Kinases - metabolism
Molecular Medicine
Myeloid Differentiation Factor 88 - metabolism
Neoplasms - immunology
Neoplasms - pathology
NF-kappa B - metabolism
Original
original-article
Pectins - pharmacology
Phenotype
Protein Transport - drug effects
Receptors, Chemokine - metabolism
Signal Transduction - drug effects
Stem Cells
T-Lymphocytes, Cytotoxic - cytology
T-Lymphocytes, Cytotoxic - drug effects
Toll-Like Receptor 4 - agonists
Toll-Like Receptor 4 - metabolism
title Rhamnogalacturonan II is a Toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T19%3A14%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rhamnogalacturonan%20II%20is%20a%20Toll-like%20receptor%204%20agonist%20that%20inhibits%20tumor%20growth%20by%20activating%20dendritic%20cell-mediated%20CD8+%20T%20cells&rft.jtitle=Experimental%20&%20molecular%20medicine&rft.au=Park,%20Sung%20Nam&rft.date=2013-02-08&rft.volume=45&rft.issue=2&rft.spage=e8&rft.epage=e8&rft.pages=e8-e8&rft.issn=2092-6413&rft.eissn=2092-6413&rft_id=info:doi/10.1038/emm.2013.14&rft_dat=%3Cproquest_pubme%3E4103392091%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1800164860&rft_id=info:pmid/23392255&rfr_iscdi=true