Circulating human basophils lack the features of professional antigen presenting cells
Recent reports in mice demonstrate that basophils function as antigen presenting cells (APC). They express MHC class II and co-stimulatory molecules CD80 and CD86, capture and present soluble antigens or IgE-antigen complexes and polarize Th2 responses. Therefore, we explored whether human circulati...
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Veröffentlicht in: | Scientific reports 2013-02, Vol.3 (1), p.1188-1188, Article 1188 |
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Sprache: | eng |
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Zusammenfassung: | Recent reports in mice demonstrate that basophils function as antigen presenting cells (APC). They express MHC class II and co-stimulatory molecules CD80 and CD86, capture and present soluble antigens or IgE-antigen complexes and polarize Th2 responses. Therefore, we explored whether human circulating basophils possess the features of professional APC. We found that unlike dendritic cells (DC) and monocytes, steady-state circulating human basophils did not express HLA-DR and co-stimulatory molecules CD80 and CD86. Basophils remained negative for these molecules following stimulation with soluble Asp f 1, one of the allergens of
Aspergillus fumigatus
; Bet v 1, the major birch allergen; TLR2-ligand or even upon IgE cross-linking. Unlike DC, Asp f 1-pulsed basophils did not promote Th2 responses as analyzed by the secretion of IL-4 in the basophil-CD4
+
T cell co-culture. Together, these results demonstrate the inability of circulating human basophils to function as professional APC. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/srep01188 |