Detection of Rotavirus Infection in Bovine Calves by RNA-PAGE and RT-PCR

A total of 128 diarrhoeic faecal samples were collected from cattle and buffalo calves from Pantnagar and Dehradun during winter months. Of the 110 cattle calves screened by RNA-PAGE, rotavirus was detected in 13 samples (11.81%) while no sample from buffalo calves was found positive. All samples we...

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Veröffentlicht in:	Indian Journal of Virology 2010-10, Vol.21 (2), p.144-147
Hauptverfasser:	Basera, Sanjay Singh, Singh, Rashmi, Vaid, N., Sharma, K., Chakravarti, S., Malik, Y. P. S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Age groups Biochemistry Biomedical and Life Sciences Cattle Cell Biology Cell culture Dairy farms Feces Genomes Health aspects Infections Life Sciences Microbiology Polymerase chain reaction Protein Structure RNA Rotavirus Short Communication Viruses
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Zusammenfassung:	A total of 128 diarrhoeic faecal samples were collected from cattle and buffalo calves from Pantnagar and Dehradun during winter months. Of the 110 cattle calves screened by RNA-PAGE, rotavirus was detected in 13 samples (11.81%) while no sample from buffalo calves was found positive. All samples were found to have long electropherotype and two distinct electropherotypes having segment variation were observed. The overall prevalence of rotavirus was 10.15% (13/128). RT-PCR targeting group specific VP6 gene confirmed Group A rotavirus in 10 out of 13 samples, while three samples remained un-groupable.
ISSN:	0970-2822 2347-3584 0974-0120 2347-3517
DOI:	10.1007/s13337-010-0017-9