Ultrasensitive Sample Quantitation via Selected Reaction Monitoring Using CITP/CZE–ESI-Triple Quadrupole MS

Ultrasensitive Sample Quantitation via Selected Reaction Monitoring Using CITP/CZE–ESI-Triple Quadrupole MS

We demonstrate the direct coupling of transient capillary isotachophoresis/capillary zone electrophoresis (CITP/CZE) with a high-sensitivity triple quadrupole mass spectrometer operating in selected reaction monitoring (SRM) mode for sample quantitation. The capability of CITP/CZE for in situ sample...

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Veröffentlicht in:Analytical Chemistry, 84(23):10395-10403 84(23):10395-10403, 2012-12, Vol.84 (23), p.10395-10403
Hauptverfasser: Wang, Chenchen, Lee, Cheng S, Smith, Richard D, Tang, Keqi
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Animals
Biochemistry
Cattle
Chemical reactions
Chemistry
Chromatographic methods and physical methods associated with chromatography
Electrophoresis, Capillary
Environmental Molecular Sciences Laboratory
Exact sciences and technology
Humans
Isotachophoresis
Mass spectrometry
Monitoring systems
Other chromatographic methods
Peptide Fragments - analysis
Reproducibility of Results
Serum Albumin, Bovine - analysis
Signal to noise ratio
Spectrometric and optical methods
Spectrometry, Mass, Electrospray Ionization
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Zusammenfassung:We demonstrate the direct coupling of transient capillary isotachophoresis/capillary zone electrophoresis (CITP/CZE) with a high-sensitivity triple quadrupole mass spectrometer operating in selected reaction monitoring (SRM) mode for sample quantitation. The capability of CITP/CZE for in situ sample enrichment and separation has been shown to significantly improve the analytical figures of merit. A linear dynamic range spanning 4 orders of magnitude was observed. An average signal-to-noise ratio (S/N) of 49.6 was observed for 50 amol of targeted peptide in the presence of a complex and much more abundant bovine serum albumin (BSA) digest. Correlation of variation (CV) of
ISSN:0003-2700
1520-6882
DOI:10.1021/ac302616m