Mass spectrometric quantification of MIF-1 in mouse brain by multiple reaction monitoring
MIF-1 (Pro-Leu-Gly-NH 2) has potent therapeutic effects in depression and Parkinson's disease, but its CNS sites of production are not yet clear. In this study, the concentration of MIF-1 in different brain regions was measured by the multiple reaction monitoring technique on a 4000 QTRAP mass...
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Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2009-07, Vol.30 (7), p.1276-1281 |
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Sprache: | eng |
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Zusammenfassung: | MIF-1 (Pro-Leu-Gly-NH
2) has potent therapeutic effects in depression and Parkinson's disease, but its CNS sites of production are not yet clear. In this study, the concentration of MIF-1 in different brain regions was measured by the multiple reaction monitoring technique on a 4000 QTRAP mass spectrometer. The limit of quantification was 300
fg of MIF-1, and limit of detection was 60
fg. The low molecular weight fractions of tissue homogenates from different regions of mouse brain were analyzed. The concentration of MIF-1 ranged from 22
±
3
fg/μg protein in cerebral cortex to 930
±
60
fg/μg protein in the hypothalamus. Moderate concentrations were also detected in all other regions tested, including the striatum, thalamus, and hippocampus. By incubation of stable isotope-labeled oxytocin with tissue preparations, it was also confirmed that oxytocin at least partially contributed to the production of MIF-1 in the hypothalamus by action of peptidases. Regional differences were also found. The results are the first to show the ultrasensitive quantification of MIF-1 in different brain regions, and support the neuromodulatory actions of MIF-1 in the striatum. |
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ISSN: | 0196-9781 1873-5169 |
DOI: | 10.1016/j.peptides.2009.04.004 |