Colorimetric Cu2+ Detection Using DNA-Modified Gold-Nanoparticle Aggregates as Probes and Click Chemistry

Colorimetric Cu2+ Detection Using DNA-Modified Gold-Nanoparticle Aggregates as Probes and Click Chemistry

The colorimetric detection of Cu2+ is demonstrated using DNA‐modified gold nanoparticle (Au NP) aggregates as probes and a copper‐catalyzed click reaction to initiate the cross‐coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chem...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2010-03, Vol.6 (5), p.623-626
Hauptverfasser: Xu, Xiaoyang, Daniel, Weston L., Wei, Wei, Mirkin, Chad A.
Format: Artikel
Sprache:eng
Schlagworte:
click chemistry
colorimetric detection
copper
DNA
nanoparticle conjugates
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Zusammenfassung:The colorimetric detection of Cu2+ is demonstrated using DNA‐modified gold nanoparticle (Au NP) aggregates as probes and a copper‐catalyzed click reaction to initiate the cross‐coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200901691